Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, Missouri, USA.
Fertil Steril. 2010 Nov;94(6):2191-6. doi: 10.1016/j.fertnstert.2009.12.073. Epub 2010 Feb 19.
To evaluate the function of human ovarian transplants.
Follow a series of fresh ovarian transplants for up to 5 years, and compare fresh and frozen ovarian tissue transplantation.
Tertiary referral community hospital.
PATIENT(S): Nine women with premature ovarian failure who received an ovary donated from a monozygotic twin sibling, and 16 young cancer patients undergoing ovarian cryopreservation. Two of the transplant recipients were cancer survivors rendered sterile by their therapy.
INTERVENTION(S): Fresh ovary transplantation between monozygotic twin sisters, as well as transplantation of previously frozen ovarian tissue, and study of cryopreserved tissue in cancer patients.
MAIN OUTCOME MEASURE(S): Return of normal menstrual cycling, hormone levels, pregnancy, healthy babies, duration of transplant function, and ovarian tissue evaluation.
RESULT(S): Normal serum FSH and regular menstrual cycles returned by 5 months after surgery in all cases, both fresh and frozen. Fourteen spontaneous pregnancies were established leading to eight healthy live births and two healthy ongoing conceptions. All three frozen tissue transplants conceived spontaneously, one delivered, and two were ongoing. Oocyte survival with slow freezing was 42% and after vitrification 89%.
CONCLUSION(S): Ovarian transplantation in humans is a robust procedure, even after cryopreservation, and vitrification might prove to be more effective than slow freezing.
评估人类卵巢移植的功能。
对一系列新鲜卵巢移植进行长达 5 年的随访，并比较新鲜和冷冻卵巢组织移植。
三级转诊社区医院。
9 名患有卵巢早衰的女性，她们接受了来自同卵双胞胎姐妹的卵巢捐赠，以及 16 名接受卵巢冷冻保存的年轻癌症患者。其中 2 名移植受者是因治疗而导致不育的癌症幸存者。
同卵双胞胎姐妹之间的新鲜卵巢移植，以及先前冷冻的卵巢组织移植，以及对癌症患者冷冻保存组织的研究。
正常月经周期恢复、激素水平、妊娠、健康婴儿、移植功能持续时间和卵巢组织评估。
所有新鲜和冷冻卵巢移植的患者在手术后 5 个月内均恢复了正常的血清 FSH 和规律的月经周期。14 例自发性妊娠导致 8 例健康活产和 2 例健康继续妊娠。所有 3 例冷冻组织移植均自发妊娠，1 例分娩，2 例继续妊娠。慢速冷冻的卵母细胞存活率为 42%，玻璃化冷冻的存活率为 89%。
人类卵巢移植是一种强大的程序，即使在冷冻保存后也是如此，玻璃化冷冻可能比慢速冷冻更有效。
