Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
Theriogenology. 2010 Jun;73(9):1244-56. doi: 10.1016/j.theriogenology.2009.12.004. Epub 2010 Feb 19.
Prostaglandins (PGs) play a pivotal role in maternal recognition of pregnancy and implantation in pigs. In the present study, PGE(2), PGF(2alpha), and PGFM (PGF(2alpha) metabolite) content, as well as PGE(2) synthase (mPGES-1) and PGF(2alpha) synthase (PGFS) expression was investigated in early pregnant gilts with natural (n=21) and PMSG/hCG-stimulated (n=19) estrus. Endometrial tissue samples, uterine luminal flushings (ULFs), and blood serum were collected on days 10-11, 12, and 15 after insemination. Additionally, day 15 conceptuses were collected for mPGES-1 and PGFS protein expression. Effect of estrus induction was observed on day 15 of pregnancy, when the content of PGE(2) in the uterine lumen was fourfold lower in gonadotropin-stimulated gilts in comparison to controls (P<0.001). Decreased PGE(2) content in ULFs of gonadotropin-treated pigs was preceded by lower endometrial mPGES-1 gene expression in hormonally-stimulated animals in comparison to control gilts (P<0.01). On the other hand, estrus induction with PMSG/hCG resulted in higher PGE(2) accumulation in the endometrial tissue on day 15 of pregnancy (P<0.01). Furthermore, PGF(2alpha) content in the endometrium and PGFM levels in blood serum were lower in gonadotropin-treated gilts, especially on day 12 after insemination when compared to control gilts (P<0.01). Finally, PGFS expression in day 15 conceptuses was decreased in animals with hormonally-induced estrus. We conclude that PMSG/hCG stimulation of prepubertal gilts to induce estrus results in changes of PG production and secretion during early pregnancy, which, in turn, may affect conceptus development, implantation, and the course of pregnancy.
前列腺素（PGs）在猪的母体识别妊娠和植入中起着关键作用。在本研究中，我们研究了自然发情（n=21）和 PMSG/hCG 刺激发情（n=19）的发情母猪在发情后第 10-11、12 和 15 天的早期怀孕时 PGE（2）、PGF（2alpha）和 PGFM（PGF（2alpha）代谢物）含量以及 PGE（2）合酶（mPGES-1）和 PGF（2alpha）合酶（PGFS）的表达。在发情后第 15 天还收集了子宫腔冲洗液（ULFs）和血清样本，以收集 mPGES-1 和 PGFS 蛋白表达。发情诱导的影响在怀孕第 15 天观察到，与对照组相比，促性腺激素刺激的母猪子宫腔中的 PGE（2）含量低四倍（P<0.001）。与对照组母猪相比，在促性腺激素处理的动物中，发情诱导后子宫内膜 mPGES-1 基因表达降低，导致 ULFs 中 PGE（2）含量降低（P<0.01）。另一方面，用 PMSG/hCG 诱导发情导致怀孕第 15 天子宫内膜中 PGE（2）的积累增加（P<0.01）。此外，在促性腺激素处理的母猪中，PGF（2alpha）在子宫内膜中的含量和 PGFM 在血清中的水平较低，尤其是在发情后第 12 天与对照组母猪相比（P<0.01）。最后，在发情诱导的动物中，第 15 天胚胎的 PGFS 表达减少。我们得出结论，用 PMSG/hCG 刺激青春期前的母猪发情会导致早期妊娠期间 PG 产生和分泌发生变化，这反过来又可能影响胚胎发育、着床和妊娠过程。
