Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland.
Int J Mol Sci. 2024 Oct 16;25(20):11102. doi: 10.3390/ijms252011102.
Fatty acids (FAs) are important for cell membrane composition, eicosanoid synthesis, and metabolic processes. Membrane proteins that facilitate FA transport into cells include FA translocase (also known as CD36) and FA transporter proteins (encoded by genes). The present study aimed to examine expression profiles of FA transporters in the endometrium of cyclic and early pregnant gilts on days 3 to 20 after estrus and the possible regulation by conceptus signals and polyunsaturated FAs (PUFAs). The effect of PUFAs on prostaglandin (PG) synthesis and transcript abundance of genes related to FA action and metabolism, angiogenesis, and immune response was also determined. Day after estrus and reproductive status of animals affected FA transporter expression, with greater levels of CD36, SLC27A1, and SLC27A4 observed in pregnant than in cyclic gilts. Conceptus-conditioned medium and/or estradiol-17β stimulated SLC27A1 and CD36 expression. Among PUFAs, linoleic acid decreased and mRNA expression, while arachidonic, docosahexaenoic, and eicosapentaenoic acids increased transcript abundance. Moreover, arachidonic acid stimulated , , and expression and increased PGE2 and PGI2 secretion. In turn, α-linolenic acid up-regulated and mRNA expression. These results indicate the presence of an active transport of FAs in the porcine endometrium and the role of PUFAs as modulators of the uterine activity during conceptus implantation.
脂肪酸（FAs）对于细胞膜组成、类二十烷酸合成和代谢过程都很重要。促进 FA 进入细胞的膜蛋白包括 FA 转位酶（也称为 CD36）和 FA 转运蛋白（由 基因编码）。本研究旨在检测发情后第 3 至 20 天的周期性和早期妊娠母猪子宫内膜中 FA 转运蛋白的表达谱，以及胚胎信号和多不饱和脂肪酸（PUFAs）对其的可能调控作用。还确定了 PUFAs 对前列腺素（PG）合成以及与 FA 作用和代谢、血管生成和免疫反应相关基因的转录丰度的影响。发情后天数和动物的生殖状态影响 FA 转运蛋白的表达，与周期性母猪相比，妊娠母猪的 CD36、SLC27A1 和 SLC27A4 水平更高。胚胎条件培养基和/或雌二醇-17β刺激 SLC27A1 和 CD36 的表达。在 PUFAs 中，亚油酸降低了 和 mRNA 的表达，而花生四烯酸、二十二碳六烯酸和二十碳五烯酸增加了 的转录丰度。此外，花生四烯酸刺激 、 和 的表达并增加 PGE2 和 PGI2 的分泌。反过来，α-亚麻酸上调 和 mRNA 的表达。这些结果表明，在猪的子宫内膜中存在 FA 的主动转运，并且 PUFAs 作为胚胎植入期间子宫活动的调节剂发挥作用。
