Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Theriogenology. 2011 Dec;76(9):1684-94. doi: 10.1016/j.theriogenology.2011.06.034. Epub 2011 Sep 3.
The purpose of the study was to investigate an effect of estrus synchronization with prostaglandin (PG) F(2α) and PMSG/hCG on WNT4, WNT5A, WNT7A, β-catenin (CTNNB1) and E-cadherin (CDH1) gene expression. The weight of the uterus, morphometrical parameters of the endometrium and the number of CL were recorded. The analysis of estradiol (E(2)), prostaglandin (PG) F(2α) and E(2) content in the uterine luminal flushings (ULFs) and progesterone (P(4)) level in the blood serum were conducted. RNA was isolated from endometrial, luteal and embryonic tissue of pregnant non-synchronized (Control; n = 15) and pregnant synchronized (PGF(2α)/PMSG/hCG; n = 15) pigs. Whereas there was no change in uterine weight, differences in height of endometrial surface and glandular epithelium were found. However, height of the endometrium, number of the glands and capillaries were unaffected. The total number of the CLs was higher (P < 0.05) in animals treated with PGF(2α)/PMSG/hCG. The amount of E(2) and P(4) was lower (P < 0.05, P < 0.001, respectively) in pregnant gilts administrated with PGF(2α)/PMSG/hCG. The concentration of PGF(2α) in ULFs was not affected by hormonal management, while PGE(2) was higher (P < 0.01) in hormonally in comparison to non-hormonally treated pigs. The content of WNT4 mRNA in conceptuses increased on particular Days studied in Control and PGF(2α)/PMSG/hCG administered animals. WNT7A and CTNNB1 were affected by PGF(2α)/PMSG/hCG treatment in both conceptuses (P < 0.001, P < 0.05) and endometrial tissue (P < 0.001, P < 0.01). The PGF(2α)/PMSG/hCG treatment resulted in elevated expression of WNT4 (P < 0.001) and CTNNB1 (P < 0.05) in luteal tissue in comparison to the Control gilts. Moreover, luteal amount of WNT5A mRNA was higher in PGF(2α)/PMSG/hCG animals in comparison to the Control group (P < 0.05). Presented data show that exogenous hormones administration can affect gene expression in the porcine reproductive tract and embryo.
本研究旨在探讨前列腺素（PG）F(2α)和 PMSG/hCG 对发情同步对 WNT4、WNT5A、WNT7A、β-连环蛋白（CTNNB1）和 E-钙粘蛋白（CDH1）基因表达的影响。记录子宫重量、子宫内膜形态参数和黄体数。分析子宫冲洗液（ULFs）中的雌二醇（E(2)）、前列腺素（PG）F(2α)和 E(2)含量以及血清中的孕酮（P(4)）水平。从非同步发情（对照；n = 15）和同步发情（PGF(2α)/PMSG/hCG；n = 15）猪的子宫内膜、黄体和胚胎组织中分离 RNA。尽管子宫重量没有变化，但发现子宫内膜表面和腺上皮的高度存在差异。然而，子宫内膜、腺体和毛细血管的数量没有受到影响。用 PGF(2α)/PMSG/hCG 处理的动物的 CL 总数更高（P < 0.05）。用 PGF(2α)/PMSG/hCG 处理的妊娠母猪的 E(2)和 P(4)水平较低（P < 0.05，P < 0.001）。ULFs 中 PGF(2α)的浓度不受激素管理的影响，而与非激素处理的猪相比，PGF(2α)/PMSG/hCG 处理的猪中 PGE(2)更高（P < 0.01）。在对照和 PGF(2α)/PMSG/hCG 处理的动物中，WNT4 mRNA 的含量在研究的特定天数增加。WNT7A 和 CTNNB1 受 PGF(2α)/PMSG/hCG 处理的影响，无论是在胚胎（P < 0.001，P < 0.05）还是子宫内膜组织（P < 0.001，P < 0.01）中。PGF(2α)/PMSG/hCG 处理导致黄体组织中 WNT4（P < 0.001）和 CTNNB1（P < 0.05）的表达升高与对照母猪相比。此外，与对照组相比，PGF(2α)/PMSG/hCG 动物的黄体 WNT5A mRNA 量更高（P < 0.05）。本研究表明，外源性激素的给予可能会影响猪生殖道和胚胎的基因表达。
