Superior Institute of Biomedical Sciences, State University of Ceará, Av. Paranjana 1700, 60740-000 Fortaleza, Ceará, CE, Brazil.
Phytomedicine. 2010 Aug;17(10):721-5. doi: 10.1016/j.phymed.2010.01.015. Epub 2010 Feb 19.
Plants of the genus Croton have been used extensively in the northeast of Brazil for treating various clinical conditions. Previous studies have demonstrated that the essential oil of some specimens of Croton sp. have a relaxing effect on tracheal smooth muscle. Our study aimed to characterize the effects of Croton sonderianus essential oil samples, collected at 1:00 pm (EO-13) and 9:00 pm (EO-21), on rat tracheal smooth muscle. The two samples were submitted to gas chromatography (GC) and mass spectrometry (MS) analysis to identify their components. Rat tracheal smooth muscle strips were used to assess the biological activity. The major constituents of EO-21 were: spathulenol (18.32%), beta-caryophyllene (14.58%) and caryophyllene oxide (8.54%) and the major constituents of EO-13 were bicyclogermacrene (16.29%), beta-phellandrene (15.42%) and beta-caryophyllene (13.82%). These samples showed an antispasmodic effect on tracheal smooth muscle strips pre-contracted with high K+ concentration (80 mM) or with acetylcholine. EO-21 increased baseline tonus while EO-13 provoked a decrease. These results demonstrated that EO-13 and EO-21 have different chemical composition and showed myorelaxant activity. In conclusion, EO-13 and EO-21 may have potential therapeutic use in the treatment of bronchospasm.
巴西东北部广泛使用大戟属植物来治疗各种临床病症。先前的研究表明，一些大戟属植物的挥发油对气管平滑肌有松弛作用。我们的研究旨在研究下午 1 点（EO-13）和晚上 9 点（EO-21）采集的大戟属桑德氏种挥发油样品对大鼠气管平滑肌的作用。这两个样本都经过气相色谱（GC）和质谱（MS）分析，以鉴定其成分。使用大鼠气管平滑肌条来评估生物活性。EO-21 的主要成分是：扇醇（18.32％），β-石竹烯（14.58％）和石竹烯氧化物（8.54％），EO-13 的主要成分是双环倍半萜烯（16.29％），β-水芹烯（15.42％）和β-石竹烯（13.82％）。这些样品对高钾浓度（80 mM）或乙酰胆碱预收缩的气管平滑肌条显示出抗痉挛作用。EO-21 增加基础张力，而 EO-13 则引起张力降低。这些结果表明，EO-13 和 EO-21 具有不同的化学成分，并具有肌松活性。总之，EO-13 和 EO-21 可能在治疗支气管痉挛方面具有潜在的治疗用途。
