Division of Head and Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Otolaryngol Head Neck Surg. 2010 Mar;142(3):438-40. doi: 10.1016/j.otohns.2009.11.020.
Tissue engineering may provide a treatment for severe vocal fold scars. This study quantifies mechanical properties and demonstrates vibration of a tissue-engineered vocal fold cover replacement.
Tissue-engineered constructs were produced from fibrin and adipose-derived stem cells. Optimized bilayered constructs contained epithelial and mesenchymal cell phenotypes in a stratified geometry. For comparison, homogeneous constructs did not have epithelial differentiation. Elastic modulus was determined using indentation. Immunohistochemical labeling for type I collagen was performed. A bilayered construct was also tested in phonation in an excised larynx model.
Bilayered vocal fold cover replacements had indentation moduli similar to human vocal fold covers (mean construct modulus 6.8 kPa). Collagen deposition occurred in the middle of the construct. Homogeneous constructs had a mean modulus of 8.3 kPa, and collagen was concentrated at the surface. An excised larynx with unilateral vocal fold cover replacement phonated and exhibited mucosal waves at physiologic airflow.
Bilayered tissue-engineered constructs were produced that exhibited indentation modulus, microstructure, and vibration similar to that exhibited by human vocal fold covers.
组织工程可能为严重声带瘢痕提供一种治疗方法。本研究定量评估了组织工程声带覆盖物替代物的力学性能并展示了其振动情况。
采用纤维蛋白和脂肪来源的干细胞制备组织工程构建物。优化的双层构建物具有上皮和间充质细胞表型，呈层状结构。为进行比较，均质构建物没有上皮分化。采用压痕法测定弹性模量。进行 I 型胶原的免疫组织化学标记。还在离体喉模型中对双层构建物进行了发音测试。
双层声带覆盖物替代物的压痕模量与人体声带覆盖物相似（构建物平均模量 6.8kPa）。在构建物的中部发生了胶原蛋白沉积。均质构建物的平均模量为 8.3kPa，且胶原蛋白集中在表面。单侧声带覆盖物替代物的离体喉可发声，并在生理气流下显示黏膜波。
成功制备了双层组织工程构建物，其压痕模量、微观结构和振动与人体声带覆盖物相似。
