Research Service, Department of Veteran's Affairs Medical Center, Los Angeles, California; Department of Head and Neck Surgery, David Geffen School of Medicine at University of California, Los Angeles, California.
Laryngoscope. 2015 Feb;125(2):406-11. doi: 10.1002/lary.24924. Epub 2014 Sep 12.
OBJECTIVES/HYPOTHESIS: An animal vocal fold replacement model is needed to investigate treatments for vocal fold scarring. We developed a rabbit surgical model, hypothesizing that orthotopic vocal fold cover implants would attach and survive. We further hypothesized that superficial scarring would be limited, allowing unimpeded vibration.
Translational research: animal surgical study.
Rabbit vocal fold covers were excised and immediately reimplanted. After 4 weeks, rabbits were phonated and vibration was recorded with high-speed videography. Larynges were then excised, elastic moduli measured by indentation, and covers sectioned for histology.
Five of six rabbits survived. Phonation was achieved in all, with mucosal waves evident. Elastic modulus did not differ significantly from contralateral uninjured control vocal folds. Histology demonstrated epithelial integrity, partial preservation of elastic fibers, and variable degrees of collagen deposition.
Vocal fold cover implantation in rabbits is feasible, and grafts survived. Attachment onto the thyroarytenoid muscle prevented excessive scarring, maintained tissue mechanics, and preserved mucosal vibration.
NA.
目的/假设：需要一种动物声带替代模型来研究治疗声带瘢痕的方法。我们开发了一种兔手术模型，假设原位声带覆盖植入物可以附着和存活。我们进一步假设，浅层瘢痕会受到限制，从而允许振动不受阻碍。
转化研究：动物手术研究。
兔声带覆盖物被切除并立即再植入。4 周后，兔子进行发声，高速录像记录振动。然后切除喉，通过压痕测量弹性模量，并对覆盖物进行切片进行组织学检查。
6 只兔子中有 5 只存活。所有兔子均能发声，可见黏膜波。弹性模量与对侧未受伤的对照声带无显著差异。组织学显示上皮完整，部分弹性纤维保存，胶原沉积程度不同。
兔声带覆盖物植入是可行的，移植物存活。附着在杓状软骨肌上可防止过度瘢痕形成，维持组织力学，并保持黏膜振动。
无。
