Department of Genetics, Stellenbosch University, Matieland, 7602, South Africa.
Virology. 2010 May 10;400(2):157-63. doi: 10.1016/j.virol.2010.01.023. Epub 2010 Feb 20.
Double stranded RNA, isolated from 44 pooled randomly selected vines from a diseased South African vineyard, has been used in a deep sequencing analysis to build a census of the viral population. The dsRNA was sequenced in an unbiased manner using the sequencing-by-synthesis technology offered by the Illumina Genome Analyzer II and yielded 837 megabases of metagenomic sequence data. Four known viral pathogens were identified. It was found that Grapevine leafroll-associated virus 3 (GLRaV-3) is the most prevalent species, constituting 59% of the total reads, followed by Grapevine rupestris stem pitting-associated virus and Grapevine virus A. Grapevine virus E, a virus not previously reported in South African vineyards, was identified in the census. Viruses not previously identified in grapevine were also detected. The second most prevalent virus detected was a member of the Chrysoviridae family similar to Penicillium chrysogenum virus. Sequences aligning to two other mycoviruses were also detected.
从南非一个患病葡萄园的 44 株随机选择的葡萄藤中分离出的双链 RNA 已被用于深度测序分析，以构建病毒群体的普查。双链 RNA 采用 Illumina Genome Analyzer II 提供的测序合成技术进行无偏测序，产生了 8.37 兆碱基对的宏基因组序列数据。鉴定出了四种已知的病毒病原体。研究发现，葡萄卷叶伴随病毒 3（GLRaV-3）是最普遍的物种，占总读数的 59%，其次是葡萄根瘤蚜茎陷点伴随病毒和葡萄病毒 A。在普查中发现了以前在南非葡萄园未报告过的葡萄病毒 E。还检测到了以前在葡萄中未鉴定出的病毒。检测到的第二大常见病毒是类似于青霉素病毒的 Chrysoviridae 家族成员。还检测到了与两种其他真菌病毒序列相匹配的序列。
