Istituto di Virologia Vegetale del C.N.R., Strada delle Cacce 73, Turin, Italy.
Virology. 2010 Dec 5;408(1):49-56. doi: 10.1016/j.virol.2010.09.001. Epub 2010 Sep 26.
Virus-derived short interfering RNAs (vsiRNAs) isolated from grapevine V. vinifera Pinot Noir clone ENTAV 115 were analyzed by high-throughput sequencing using the Illumina Solexa platform. We identified and characterized vsiRNAs derived from grapevine field plants naturally infected with different viruses belonging to the genera Foveavirus, Maculavirus, Marafivirus and Nepovirus. These vsiRNAs were mainly of 21 and 22 nucleotides (nt) in size and were discontinuously distributed throughout Grapevine rupestris stem-pitting associated virus (GRSPaV) and Grapevine fleck virus (GFkV) genomic RNAs. Among the studied viruses, GRSPaV and GFkV vsiRNAs had a 5' terminal nucleotide bias, which differed from that described for experimental viral infections in Arabidopsis thaliana. VsiRNAs were found to originate from both genomic and antigenomic GRSPaV RNA strands, whereas with the grapevine tymoviruses GFkV and Grapevine Red Globe associated virus (GRGV), the large majority derived from the antigenomic viral strand, a feature never observed in other plant-virus interactions.
从感染不同病毒的葡萄品种 Pinot Noir 克隆 ENTAV 115 中分离出的病毒衍生的小干扰 RNA（vsiRNA），采用 Illumina Solexa 平台进行高通量测序分析。我们鉴定并描述了来源于田间受不同病毒感染的葡萄植株的 vsiRNA，这些病毒属于 Foveavirus、Maculavirus、Marafivirus 和 Nepovirus 属。这些 vsiRNA 主要为 21 和 22 个核苷酸（nt）大小，在葡萄岩榴潜隐病毒（GRSPaV）和葡萄斑点病毒（GFkV）基因组 RNA 中呈不连续分布。在所研究的病毒中，GRSPaV 和 GFkV vsiRNA 具有 5'端核苷酸偏好性，这与在拟南芥中进行的实验性病毒感染描述不同。vsiRNA 来源于 GRSPaV 的基因组和抗原基因组 RNA 链，而对于葡萄番茄花叶病毒 GFkV 和葡萄红地球相关病毒（GRGV），大多数来源于抗原基因组病毒链，这是在其他植物-病毒相互作用中从未观察到的特征。
