北极甲烷杆菌（Methanobacterium arcticum）sp. nov.，一种来自全新世北极永冻层的产甲烷古菌。
Methanobacterium arcticum sp. nov., a methanogenic archaeon from Holocene Arctic permafrost.
机构信息
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russian Federation.
Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russian Federation.
出版信息
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):144-147. doi: 10.1099/ijs.0.021311-0. Epub 2010 Feb 19.
A mesophilic, non-motile, hydrogenotrophic, rod-shaped methanogen, designated M2(T), was isolated from Holocene permafrost sediments of the Kolyma lowland in the Russian Arctic. Cells were 3-6 μm long and 0.45-0.5 μm wide. Strain M2(T) grew on H(2)/CO(2) and formate. Optimum conditions for growth were 37°C, pH 6.8-7.2 and 0.1 M NaCl. The DNA G+C content was 38.1 mol%. On the basis of 16S rRNA gene sequence comparison with known methanogens, strain M2(T) was affiliated with the genus Methanobacterium and was most closely related to Methanobacterium veterum MK4(T) and Methanobacterium bryantii DSM 863(T) (both 99 % 16S rRNA gene sequence similarity). However, no significant DNA-DNA relatedness was observed between strain M2(T) and these type strains. We propose that strain M2(T) represents a novel species, with the name Methanobacterium arcticum sp. nov., with type strain M2(T) (=DSM 19844(T) =VKM B-2371(T)).
一种中温、非运动、产氢、杆状甲烷菌，命名为 M2(T)，从俄罗斯北极科雷马低地的全新世永冻土沉积物中分离得到。细胞长 3-6μm，宽 0.45-0.5μm。菌株 M2(T)可以在 H(2)/CO(2)和甲酸盐上生长。最佳生长条件为 37°C、pH 值 6.8-7.2 和 0.1 M NaCl。DNA G+C 含量为 38.1 mol%。基于与已知产甲烷菌的 16S rRNA 基因序列比较，菌株 M2(T)属于甲烷杆菌属，与 Methanobacterium veterum MK4(T)和 Methanobacterium bryantii DSM 863(T)最为密切相关（两者的 16S rRNA 基因序列相似性均为 99%）。然而，菌株 M2(T)与这些模式株之间没有观察到显著的 DNA-DNA 相关性。我们建议将菌株 M2(T)代表一个新的物种，命名为北极甲烷杆菌（Methanobacterium arcticum）sp. nov.，其模式菌株为 M2(T)（=DSM 19844(T)=VKM B-2371(T)）。
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