激酶抑制剂类似物1NM-PP1的使用揭示了弓形虫CDPK1在入侵步骤中的作用。
Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxoplasma gondii CDPK1 in the invasion step.
作者信息
Sugi Tatsuki, Kato Kentaro, Kobayashi Kyosuke, Watanabe Shumpei, Kurokawa Hitomi, Gong Haiyan, Pandey Kishor, Takemae Hitoshi, Akashi Hiroomi
机构信息
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
Eukaryot Cell. 2010 Apr;9(4):667-70. doi: 10.1128/EC.00351-09. Epub 2010 Feb 19.
Abstract
Toxoplasma gondii CDPK1 (TgCDPK1) was found to be the target of the toxoplasmocidal compound 1NM-PP1. When TgCDPK1 was mutated at position 128 from glycine to methionine, resistance was gained. Inhibition of gliding motility without inhibition of micronemal secretion by 1NM-PP1 suggests a function for TgCDPK1 in gliding motility.
摘要
弓形虫钙依赖蛋白激酶1（TgCDPK1）被发现是抗弓形虫化合物1NM-PP1的作用靶点。当TgCDPK1第128位的甘氨酸突变为甲硫氨酸时，产生了抗性。1NM-PP1抑制滑行运动但不抑制微线体分泌，这表明TgCDPK1在滑行运动中发挥作用。
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