Smoot E C, Kucan J O, Roth A, Mody N, Debs N
Southern Illinois University School of Medicine, Springfield.
Plast Reconstr Surg. 1991 May;87(5):917-24. doi: 10.1097/00006534-199105000-00017.
The use of cultured human keratinocytes in an in vitro comparison of topical antibacterial toxicity for epithelial cells was examined. The complement of three assessments allows testing of epithelial migration, growth, and survival. The three assessments included (1) flow cytometry for determination of cell survival, (2) a comparison of confluent cell culture growth after antibacterial exposures, and (3) an evaluation of cell migration using a technique of dermal explants to study radial migration. A comparative ranking of the toxicities of the various topical antibacterials was determined with the three assessments. This has confirmed anecdotal reports that many of the topical antibacterials are cell-toxic and may inhibit wound healing. This information can be directly extrapolated to the clinical setting, unlike many of the animal data for wound healing that currently exist.
研究了在体外比较局部抗菌剂对上皮细胞毒性时使用培养的人角质形成细胞的情况。三项评估的组合可以测试上皮细胞的迁移、生长和存活情况。这三项评估包括：（1）通过流式细胞术测定细胞存活率；（2）比较抗菌剂处理后汇合细胞培养物的生长情况；（3）使用皮肤外植体技术评估细胞迁移以研究径向迁移。通过这三项评估确定了各种局部抗菌剂毒性的比较排名。这证实了一些传闻报道，即许多局部抗菌剂具有细胞毒性，可能会抑制伤口愈合。与目前存在的许多关于伤口愈合的动物数据不同，这些信息可以直接外推到临床环境中。
