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基于透射电子显微镜和X射线微分析联用的方法，对培养的人角质形成细胞摄取包裹磺胺嘧啶银的完整脂质体的直接证据。

Direct evidence for uptake of intact liposomes encapsulating silver sulfadiazine by cultured human keratinocytes based on combined transmission electron microscopy and X-ray microanalysis.

作者信息

Schaller M, Wurm R, Korting H C

机构信息

Department of Dermatology, Ludwig Maximilians University, Munich, Germany.

出版信息

Antimicrob Agents Chemother. 1997 Mar;41(3):717-9. doi: 10.1128/AAC.41.3.717.

DOI:10.1128/AAC.41.3.717
PMID:9056023
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC163781/
Abstract

Cultured human keratinocytes were exposed to liposomally encapsulated silver sulfadiazine, free silver sulfadiazine, silver sulfadiazine cream, and a corresponding vehicle for 5 min to 24 h. Phagocytosis of intact liposomes by keratinocytes was demonstrated in vitro by combined X-ray microanalysis and electron microscopy. Silver as an active part of the antimicrobial served as an electron-dense marker.

摘要

将培养的人角质形成细胞暴露于脂质体包裹的磺胺嘧啶银、游离磺胺嘧啶银、磺胺嘧啶银乳膏以及相应的赋形剂中5分钟至24小时。通过结合X射线微量分析和电子显微镜在体外证实了角质形成细胞对完整脂质体的吞噬作用。作为抗菌活性成分的银充当电子致密标记物。

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Direct evidence for uptake of intact liposomes encapsulating silver sulfadiazine by cultured human keratinocytes based on combined transmission electron microscopy and X-ray microanalysis.基于透射电子显微镜和X射线微分析联用的方法，对培养的人角质形成细胞摄取包裹磺胺嘧啶银的完整脂质体的直接证据。
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本文引用的文献

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Gluteal pigmentation after intramuscular iron injections.肌肉注射铁剂后臀部色素沉着。
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Interaction of cultured human keratinocytes with liposomes encapsulating silver sulphadiazine: proof of the uptake of intact vesicles.培养的人角质形成细胞与包裹磺胺嘧啶银的脂质体的相互作用：完整囊泡摄取的证据。
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In vitro toxicity testing for antibacterials against human keratinocytes.抗菌剂对人角质形成细胞的体外毒性测试。
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