Department of Biochemistry, Biomedical Science (WCU Program), College of Life Science and Biotechnology, Yonsei Proteome Research Center, Yonsei University, Seoul, Korea.
Proteomics. 2010 Mar;10(6):1250-5. doi: 10.1002/pmic.200900371.
As human plasma is clinically valuable, reference data from healthy donors can be a useful source for serological biomarker studies. To make a reliable protein catalog of the Korean plasma proteome, various experimental methods, such as 1-D HPLC, 2-D LC, and narrow ranged 2-DE prior to MALDI-TOF and LC-MS/MS, were used to identify unique plasma proteins in this population. To compile candidates with high confidence, two different search engines were used to select proteins with a false discovery rate of less than or equal to 1%. From this rigorous selection process, we initially identified 494 distinct Korean plasma proteins. After multilevel stepwise filtrations with stringent, identification parameters were applied to acquire plasma protein list with the maximum confidence; a total 185 distinct plasma proteins were identified and integrated into our Korean human plasma proteome project database along with several bioinformatics analysis results, including gene ontology, biological pathways, tissue expression, and disease association. This is the first publicly available single ethnic group-specific plasma proteome database (http://proteomix.org/khppp/).
由于人血浆在临床上具有重要价值，因此健康供体的参考数据可以成为血清生物标志物研究的有用来源。为了可靠地编制韩国人血浆蛋白质组图谱，使用了各种实验方法，如 1-D HPLC、2-D LC 和窄范围 2-DE 凝胶电泳，然后进行 MALDI-TOF 和 LC-MS/MS 分析，以鉴定该人群中的独特血浆蛋白。为了编译高可信度的候选蛋白，使用了两种不同的搜索引擎来选择假阳性率低于或等于 1%的蛋白。通过严格的筛选过程，我们最初鉴定了 494 种独特的韩国人血浆蛋白。经过多层次逐步过滤和严格的鉴定参数应用，我们获得了可信度最高的血浆蛋白列表，共鉴定出 185 种独特的血浆蛋白，并将其与包括基因本体、生物途径、组织表达和疾病关联在内的几个生物信息学分析结果一起整合到我们的韩国人血浆蛋白质组项目数据库中。这是第一个公开的单一特定族群的血浆蛋白质组数据库(http://proteomix.org/khppp/)。
