PROIMI-CONICET, Av. Belgrano y Pasaje Caseros, MVB, Tucumán, Argentina.
J Basic Microbiol. 2010 Feb;50(1):52-8. doi: 10.1002/jobm.200900162.
Under the current assay conditions, lipase production in mineral medium was only detected in the presence of vegetable oils, reaching the highest specific activity with olive oil. In this way, effect of different environmental conditions on fungal morphology and olive oil-induced extracellular lipases production from Aspergillus niger MYA 135 was studied. It was observed that addition of 1.0 g l(-1) FeCl(3)to the medium encouraged filamentous growth and increased the specific activity 6.6 fold after 4 days of incubation compared to the control. However, major novelty of this study was the satisfactory production of an acidic lipase at initial pH 3 of the culture medium (1.74 +/- 0.06 mU microg(-1)), since its potencial applications in food and pharmaceutical industry are highly promising.
在当前的分析条件下,仅在存在植物油的情况下才会检测到在矿物培养基中的脂肪酶生产,并且橄榄油能达到最高的比活度。通过这种方式,研究了不同环境条件对黑曲霉 MYA 135 的真菌形态和橄榄油诱导的细胞外脂肪酶生产的影响。观察到,向培养基中添加 1.0 g l(-1)FeCl(3)会促进丝状生长,并与对照相比,在孵育 4 天后将比活度提高了 6.6 倍。然而,这项研究的主要新颖之处在于在培养基初始 pH 3 时(1.74 +/- 0.06 mU microg(-1)),能够令人满意地生产出一种酸性脂肪酶,因为它在食品和制药工业中的潜在应用非常有前景。