Wyle, 1290 Hercules Drive, Houston, TX 77058, USA.
Mutat Res. 2010 Aug 14;701(1):75-9. doi: 10.1016/j.mrgentox.2010.02.007. Epub 2010 Feb 20.
Cytogenetic damage was assessed in blood lymphocytes from 16 astronauts before and after they participated in long-duration space missions of 3 months or more. The frequency of chromosome damage was measured by fluorescence in situ hybridization (FISH) chromosome painting before flight and at various intervals from a few days to many months after return from the mission. For all individuals, the frequency of chromosome exchanges measured within a month of return from space was higher than their preflight yield. However, some individuals showed a temporal decline in chromosome damage with time after flight. Statistical analysis using combined data for all astronauts indicated a significant overall decreasing trend in total chromosome exchanges with time after flight, although this trend was not seen for all astronauts and the yield of chromosome damage in some individuals actually increased with time after flight. The decreasing trend in total exchanges was slightly more significant when statistical analysis was restricted to data collected more than 220 days after return from flight. When analysis was restricted to data collected within 220 days of return from the mission there was no relationship between total exchanges and time. Translocation yields varied more between astronauts and there was only a slight non-significant decrease with time after flight that was similar for both later and earlier sampling times.
我们评估了 16 名宇航员在参与为期 3 个月或更长时间的长期太空任务前后血液淋巴细胞中的细胞遗传学损伤。飞行前和返回任务后几天到几个月的不同时间点，通过荧光原位杂交（FISH）染色体着色来测量染色体损伤的频率。对于所有个体，返回太空后一个月内测量的染色体交换频率高于飞行前的水平。然而，一些个体在飞行后随着时间的推移显示出染色体损伤的时间下降。使用所有宇航员的综合数据进行的统计分析表明，总染色体交换随着飞行后时间的推移呈显著下降趋势，尽管并非所有宇航员都出现这种趋势，并且一些个体的染色体损伤水平实际上随着飞行后时间的推移而增加。当将统计分析仅限于从飞行返回后超过 220 天收集的数据时，总交换的下降趋势更为明显。当分析仅限于从任务返回后 220 天内收集的数据时，总交换与时间之间没有关系。易位产量在宇航员之间差异更大，与后期和早期采样时间相比，只有略微非显著的随时间下降的趋势。
