Wu H, George K, Willingham V, Cucinotta F A
Radiation Biophysics Laboratory, NASA Johnson Space Center, Houston, TX 77058, USA.
Phys Med. 2001;17 Suppl 1:229-31.
If radiosensitivity is altered in a microgravity environment, it will affect the accuracy of assessing astronauts' risk from exposure to space radiation. To investigate the effects of space flight on radiosensitivity, we exposed a crewmember's blood to gamma rays at doses ranging from 0 to 3 Gy and analyzed chromosome aberrations in mitotic lymphocytes. The blood samples were collected 10 days prior to an 8-day Shuttle mission, the day the flight returned, and 14 days after the flight. After exposure, lymphocytes were stimulated to grow in media containing phytohaemagglutinin (PHA) and mitotic cells were harvested for chromosome analysis using a fluorescence in situ hybridization (FISH) with whole chromosome specific probes. The dose response of total exchanges showed no changes in the radiosensitivity after the mission.
如果在微重力环境中辐射敏感性发生改变，将会影响评估宇航员遭受太空辐射风险的准确性。为了研究太空飞行对辐射敏感性的影响，我们将一名机组人员的血液暴露于剂量范围为0至3 Gy的伽马射线下，并分析有丝分裂淋巴细胞中的染色体畸变情况。血液样本在一次为期8天的航天飞机任务前10天、飞行返回当天以及飞行后14天采集。暴露后，淋巴细胞在含有植物血凝素（PHA）的培养基中被刺激生长，有丝分裂细胞被收获，使用全染色体特异性探针通过荧光原位杂交（FISH）进行染色体分析。总交换的剂量反应显示任务后辐射敏感性没有变化。
