Oxford Brookes University, School of Social Sciences and Law, Department of Anthropology and Geography, OX3 0BP Oxford, United Kingdom.
C R Biol. 2010 Jan;333(1):11-6. doi: 10.1016/j.crvi.2009.10.003. Epub 2010 Feb 8.
For comparative primatology proper recognition of basal taxa (i.e. species) is indispensable, and in this the choice of a suitable gene with high phylogenetic resolution is crucial. For the goals of species identification in animals, the cytochrome c oxidase subunit 1 (cox1) has been introduced as standard marker. Making use of the difference in intra- and interspecific genetic variation--the DNA barcoding gap--cox1 can be used as a fast and accurate marker for the identification of animal species. For the Order Primates we compare the performance of cox1 (166 sequences; 50 nominal species) in species-identification with that of two other mitochondrial markers, 16S ribosomal RNA (412 sequences, 92 species) and cytochrome b (cob: 547 sequences, 72 species). A wide gap exist between intra- and interspecific divergences for both cox1 and cob genes whereas this gap is less apparent for 16S, indicating that rRNA genes are less suitable for species delimitation in DNA barcoding. For those species where multiple sequences are available there are significant differences in the intraspecific genetic distances between different mitochondrial markers, without, however, showing a consistent pattern. We conclude that cox1 allows accurate differentiation of species and as such DNA barcoding may have an important role to play in comparative primatology.
对于比较灵长类学，正确识别基础分类单元（即物种）是必不可少的，而在这一点上，选择一个具有高系统发育分辨率的合适基因是至关重要的。对于动物物种鉴定的目标，细胞色素 c 氧化酶亚基 1（cox1）已被引入作为标准标记。利用种内和种间遗传变异的差异——DNA 条形码间隙——cox1 可以作为鉴定动物物种的快速、准确的标记。对于灵长目目，我们比较了 cox1（166 个序列；50 个名义种）在物种鉴定中的表现，以及另外两个线粒体标记物，16S 核糖体 RNA（412 个序列，92 个种）和细胞色素 b（cob：547 个序列，72 个种）的表现。cox1 和 cob 基因的种内和种间分化之间存在很大的差距，而 16S 的差距则不那么明显，这表明 rRNA 基因不太适合 DNA 条形码中的物种划分。对于那些有多个序列的物种，不同线粒体标记物之间的种内遗传距离存在显著差异，但没有表现出一致的模式。我们得出结论，cox1 允许对物种进行准确的区分，因此 DNA 条形码在比较灵长类学中可能具有重要作用。
