College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Evid Based Complement Alternat Med. 2011;2011:978253. doi: 10.1155/2011/978253. Epub 2011 May 11.
DNA barcoding is a molecular method that uses a short standardized DNA sequence as a species identification tool. In this study, the standard 652 base-pair region of the mitochondrial cytochrome oxidase subunit I gene (COI) was sequenced in marine fish specimens captured in China. The average genetic distance was 50-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 15.742% among congeners and only 0.319% for intraspecific individuals. There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the genera Pampus in which the introgressive hybridization was detected. High efficiency of species identification was demonstrated in the present study by DNA barcoding. Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies.
DNA 条形码是一种分子方法，它使用短的标准化 DNA 序列作为物种鉴定工具。在这项研究中，对在中国捕获的海洋鱼类标本进行了线粒体细胞色素氧化酶亚基 I 基因（COI）的标准 652 碱基对区域进行了测序。种间平均遗传距离比种内高 50 倍，金氏二参数（K2P）遗传距离在同属物种中平均为 15.742%，而种内个体仅为 0.319%。除了检测到渐渗杂交的 Pampus 属之外，同种和种间比较的成对遗传变异之间没有重叠。本研究通过 DNA 条形码证明了高效的物种鉴定能力。由于存在隐种，建议设定一个假定的阈值，以加速发现新物种和生物多样性，特别是涉及研究较少的生物群。
