Department of Clinical Virology, University of Gothenburg, S-413 46 Göteborg, Sweden.
Virology. 2010 May 10;400(2):197-206. doi: 10.1016/j.virol.2010.01.032. Epub 2010 Feb 21.
Human antibodies specific for glycoprotein C (gC1) of herpes simplex virus type 1 (HSV-1) neutralized the virus infectivity and efficiently inhibited attachment of HSV-1 to human HaCaT keratinocytes and to murine mutant L cells expressing either heparan sulfate or chondroitin sulfate at the cell surface. Similar activities were observed with anti-gC1 monoclonal antibody B1C1. In addition to HaCaT and L cells, B1C1 antibody neutralized HSV-1 infectivity in simian GMK AH1 cells mildly pre-treated with heparinase III. Human anti-gC1 antibodies efficiently competed with the binding of gC1 to B1C1 antibody whose epitope overlaps a part of the attachment domain of gC1. Human anti-gC1 and B1C1 antibodies extended survival time of mice experimentally infected with HSV-1. We conclude that in HaCaT cells and in cell systems showing restricted expression of glycosaminoglycans, human and some monoclonal anti-gC1 antibodies can target the cell-binding domain of this protein and neutralize viral infectivity.
人类针对单纯疱疹病毒 1 型 (HSV-1) 糖蛋白 C (gC1) 的抗体能中和病毒的感染力，并有效地抑制 HSV-1 与人类 HaCaT 角质形成细胞和表达细胞表面硫酸乙酰肝素或硫酸软骨素的鼠突变 L 细胞的附着。抗 gC1 单克隆抗体 B1C1 也具有类似的活性。除了 HaCaT 和 L 细胞外，B1C1 抗体还能中和经肝素酶 III 轻度预处理的猴 GMK AH1 细胞中的 HSV-1 感染性。人抗 gC1 抗体能有效地与 gC1 结合 B1C1 抗体竞争，而 B1C1 抗体的表位与 gC1 的附着域的一部分重叠。人抗 gC1 和 B1C1 抗体延长了实验感染 HSV-1 的小鼠的存活时间。我们得出结论，在 HaCaT 细胞和表达糖胺聚糖受到限制的细胞系统中，人类和一些单克隆抗 gC1 抗体可以针对该蛋白的细胞结合域，并中和病毒的感染力。
