Institute of Experimental Immunology, affiliated to EUROIMMUN AG, Seekamp 31, D-23560 Luebeck, Germany.
J Virol Methods. 2010 Jun;166(1-2):42-7. doi: 10.1016/j.jviromet.2010.02.015. Epub 2010 Feb 18.
Seroreactivity to the glycoproteins C and G of herpes simplex virus type 1 (HSV-1) was compared in 310 serum samples using a Western blot assay containing a whole antigen extract of HSV-1 and an ELISA employing gC1 isolated from HSV-1. The prevalence of reactivity to gC1 was 75.8% by Western blot and 73.9% by ELISA, while antibody responses to gG1 were detected in 72.9% of sera by Western blot. An absolute correlation of 96.1% between the reactivity to gC1 and gG1 was demonstrated using the Western blot. The gC1-based ELISA correlated with Western blot detection of anti-gC1 and anti-gG1 antibodies in 95.2 and 97.7% of samples, respectively. 3.2% of all sera were reactive with gC1 in Western blot and/or ELISA, but were negative for anti-gG1. For analysis of cross-reactivity, antibodies against HSV-2, Epstein-Barr virus, varicella-zoster virus and cytomegalovirus were determined. The prevalence of antibodies against each individual virus was identical in the groups of sera reactive with gC1 or gG1. These findings indicate that gC1 and gG1 are equivalent antigenic targets for the type-specific serodiagnosis of HSV-1 infections.
使用包含 HSV-1 全抗原提取物的 Western blot 分析和从 HSV-1 分离的 gC1 的 ELISA，比较了 310 份血清样品中针对单纯疱疹病毒 1 型（HSV-1）糖蛋白 C 和 G 的血清反应性。Western blot 检测到 gC1 反应的流行率为 75.8%，ELISA 为 73.9%，而 Western blot 检测到 72.9%的血清中存在针对 gG1 的抗体反应。Western blot 显示 gC1 与 gG1 之间的反应绝对相关，相关性为 96.1%。基于 gC1 的 ELISA 与 Western blot 检测抗 gC1 和抗 gG1 抗体的相关性分别为 95.2%和 97.7%。Western blot 和/或 ELISA 中，3.2%的所有血清与 gC1 反应，但抗 gG1 为阴性。为了分析交叉反应性，测定了针对 HSV-2、Epstein-Barr 病毒、水痘-带状疱疹病毒和巨细胞病毒的抗体。在与 gC1 或 gG1 反应的血清组中，每种病毒的抗体流行率相同。这些发现表明 gC1 和 gG1 是针对 HSV-1 感染的特定类型血清诊断的等效抗原靶标。
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