Patel R M, Shah R R
Plant Tissue Culture Laboratory, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Navsari-396 450, India.
Indian J Pharm Sci. 2009 Jan;71(1):46-50. doi: 10.4103/0250-474X.51954.
Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to maintain callus on Murashige and Skoog medium supplemented with 6-benzyl amino purine and naphthalene acetic acid. Maximum callus induction was obtained on Murashige and Skoog medium incorporated with 6-benzyl amino purine (2.0-3.0 mg/l) and naphthalene acetic acid (2.0 mg/l) treatments. However, Murashige and Skoog medium containing 2.0 mg/l 6-benzyl amino purine+2.0 mg/l naphthalene acetic acid was found to be the best for callus induction. Higher regeneration frequency was noticed with Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyl amino purine+0.2 mg/l naphthalene acetic acid. Regenerated plants were rooted better on (1/4) Murashige and Skoog strength supplemented with 0.1 mg/l indole-3-butyric acid. The rooted plantlets were hardened successfully in tera care medium with 63 per cent survival rate. The developed protocol can be utilized for mass production of true to type planting material on large scale independent of season, i.e. external environmental conditions.
甜叶菊传统上通过种子、扦插或分株繁殖，存在种子材料质量和数量的限制。在本研究中，尝试采用愈伤组织培养技术来实现优质种子材料的快速植株增殖。从茎节和叶片切段对愈伤组织诱导和增殖培养基进行了标准化。在添加了6-苄基氨基嘌呤和萘乙酸的Murashige和Skoog培养基上可以维持愈伤组织。在添加了6-苄基氨基嘌呤（2.0 - 3.0毫克/升）和萘乙酸（2.0毫克/升）处理的Murashige和Skoog培养基上获得了最大的愈伤组织诱导率。然而，发现含有2.0毫克/升6-苄基氨基嘌呤 + 2.0毫克/升萘乙酸的Murashige和Skoog培养基最适合愈伤组织诱导。在添加了2.0毫克/升6-苄基氨基嘌呤 + 0.2毫克/升萘乙酸的Murashige和Skoog培养基上观察到更高的再生频率。再生植株在添加了0.1毫克/升吲哚-3-丁酸的（1/4）Murashige和Skoog强度培养基上生根更好。生根的小植株在温室护理培养基中成功驯化，成活率为63%。所开发的方案可用于大规模独立于季节（即外部环境条件）生产纯正类型的种植材料。
