Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan.
Planta Med. 1986 Aug(4):321-3. doi: 10.1055/s-2007-969165.
Shoot primordia, which were able to propagate vegetatively with a very high rate and to redifferentiate easily to new plants, were induced from shoot tips of Stevia rebaudiana Bertoni on Gamborg B5 medium containing 6-benzylaminopurine (BAP) and alpha-naphthaleneacetic acid (NAA) under light. The propagation of the shoot primordia of Stevia rebaudiana is rapid, and they are highly stable in chromosome number and karyotype. The shoot primordia can propagate at a high rate for a long time without differentiation. At any time, the shoot primordia readily developed into plantlets with shoots and roots within 2 or 3 weeks in static culture on B5 medium containing 0.02 mg/l BAP and 2% sucrose. The plantlets were transplanted to sterilized soil to grow to normal adult plants.
茎原基可进行高频率的营养繁殖并能轻易地再分化为新植株，通过光照在含 6-苄氨基嘌呤（BAP）和α-萘乙酸（NAA）的 Gamborg B5 培养基上由甜菊（Stevia rebaudiana Bertoni）茎尖诱导而来。甜菊茎原基的繁殖速度很快，其染色体数目和核型非常稳定。茎原基在长时间内可保持高频率的无性繁殖而不分化。在 B5 培养基中添加 0.02mg/l BAP 和 2%蔗糖的静态培养条件下，任何时候的茎原基都能在 2 至 3 周内迅速发育成具有茎和根的小植株。小植株可移栽到消毒土壤中生长为正常的成年植株。
