盐酸二甲双胍分析的分光光度法
Spectrophotometric method for analysis of metformin hydrochloride.
作者信息
Mubeen G, Noor Khalikha
机构信息
Al-Ameen College of Pharmacy, Hosur Road, Bangalore-560 027, India.
出版信息
Indian J Pharm Sci. 2009 Jan;71(1):100-2. doi: 10.4103/0250-474X.51947.
A simple and sensitive spectrophotometric method has been developed and validated for the estimation of metformin hydrochloride in bulk and in tablet formulation. The primary amino group of metformin hydrochloride reacts with ninhydrin in alkaline medium to form a violet colour chromogen, which is determined spectrophotometrically at 570 nm. It obeyed Beer's law in the range of 8-18 mug/ml. Percentage recovery of the drug for the proposed method ranged from 97-100% indicating no interference of the tablet excipients. The proposed method was found to be accurate and precise for routine estimation of metformin hydrochloride in bulk and from tablet dosage forms.
已开发并验证了一种简单且灵敏的分光光度法，用于测定原料药和片剂制剂中的盐酸二甲双胍。盐酸二甲双胍的伯氨基在碱性介质中与茚三酮反应形成紫色发色团，于570nm处进行分光光度测定。在8 - 18μg/ml范围内符合比尔定律。该方法对药物的回收率在97 - 100%之间，表明片剂辅料无干扰。结果表明，该方法对于常规测定原料药和片剂剂型中的盐酸二甲双胍准确且精密。
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