Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100, Torun, Poland.
Department of Physical Chemistry and Physicochemistry of Polymers, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100, Torun, Poland.
Anal Bioanal Chem. 2010 May;397(1):173-179. doi: 10.1007/s00216-010-3483-4. Epub 2010 Feb 23.
Five adrenolytic drugs have been analyzed by liquid chromatography-mass spectrometry (LC-MS). Samples were prepared by solid-phase microextraction (SPME) using polypyrrole fibers coated on stainless steel support as an adsorbent for the drugs. Adsorption efficiencies were 95% and were close for all the drugs investigated. Relative standard deviations (RSD), calculated for samples prepared in standard solutions, were in the range 2.5-13%, however RSD values for the drugs in human plasma were 2.5-4.5%. Using LC-MS the limit of detection (LOD) and the limit of quantification (LOQ) were in the ranges 0.11-0.18 and 0.39-0.54 ng mL(-1), respectively, for the five drugs.
五种肾上腺抑制剂已通过液相色谱-质谱联用（LC-MS）进行了分析。样品采用固相微萃取（SPME）法制备，以涂覆在不锈钢支撑体上的聚吡咯纤维作为药物的吸附剂。所有研究药物的吸附效率均为 95%，且非常接近。在标准溶液中制备样品时，相对标准偏差（RSD）的计算值在 2.5-13%范围内，而人血浆中药物的 RSD 值为 2.5-4.5%。使用 LC-MS，五种药物的检测限（LOD）和定量限（LOQ）分别在 0.11-0.18 和 0.39-0.54 ng mL(-1)范围内。
