Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, Torun, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Aug 15;878(24):2226-34. doi: 10.1016/j.jchromb.2010.06.033. Epub 2010 Jul 3.
In this study, polypyrrole (PPy) and polythiophene (PTh) SPME coatings and their ability to extract selected adrenolytic drugs with different physico-chemical properties from standard solutions and human plasma samples were evaluated. In measurements metoprolol, oxprenolol, mexiletine, propranolol, and propaphenon were investigated. The main parameters such as extraction time, desorption conditions and pH influence were examined. Inter-day precisions were in range 0.1-2.0%, 1.1-2.9%, 1.3-2.6%, 0.1-2.6% and 0.3-2.1% for metoprolol, oxprenolol, mexiletine, propranolol and propaphenon, respectively. Accuracies were less than 15%, which was evaluated by analyzing preparation samples of five replicates. The method was successfully applied to human plasma samples spiked with selected adrenolytic drugs. The method was linear in the concentration range from 1 to 10microg/ml for all of studied adrenolytic drugs using human plasma samples. The PTh-SPME coating displayed higher extraction efficiency towards the target analytes in comparison to PPy-SPME. The reproducibility of the extraction using polypyrrole and polythiophene fibers was confirmed by variation coefficients lower than 8% and 3%, respectively.
在这项研究中，评估了聚吡咯（PPy）和聚噻吩（PTh）SPME 涂层及其从标准溶液和人血浆样品中提取具有不同物理化学性质的选定肾上腺素能药物的能力。在测量中，研究了美托洛尔、氧烯洛尔、美西律、普萘洛尔和丙苯酮。检查了主要参数，如萃取时间、解吸条件和 pH 影响。美托洛尔、氧烯洛尔、美西律、普萘洛尔和丙苯酮的日内精密度分别在 0.1-2.0%、1.1-2.9%、1.3-2.6%、0.1-2.6%和 0.3-2.1%范围内。通过分析五个重复制备样品评估，准确度小于 15%。该方法成功应用于添加选定肾上腺素能药物的人血浆样品。使用人血浆样品，该方法在所有研究的肾上腺素能药物的浓度范围为 1 至 10μg/ml 时呈线性。与 PPy-SPME 相比，PTh-SPME 涂层对目标分析物显示出更高的萃取效率。通过低于 8%和 3%的变化系数，分别确认了使用聚苯胺和聚噻吩纤维进行萃取的重现性。
