Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
Neurochem Res. 2010 May;35(5):811-9. doi: 10.1007/s11064-010-0139-x. Epub 2010 Feb 23.
Fat-1 transgenic mice endogenously convert n-6 to n-3 polyunsaturated fatty acids (PUFA). The aims of this study were to test whether a) fish oil feeding can attain similar brain n-3 PUFA levels as the fat-1 mouse, and b) fat-1 mouse brain docosahexaenoic acid (22:6n-3; DHA) levels can be potentiated by fish oil feeding. Fat-1 mice and their wildtype littermates consumed either a 10% safflower oil (SO) or a 2% fish oil and 8% safflower oil chow (FO). Brain total lipid and phospholipid fraction fatty acids were analyzed using GC-FID. Wildtype mice fed FO chow had similar brain levels of DHA as fat-1 mice fed SO chow. Fat-1 mice fed FO chow had similar brain n-3 PUFA levels as fat-1 mice fed SO chow. In conclusion, brain levels of DHA in the fat-1 mouse can be obtained by and were not further augmented with fish oil feeding.
Fat-1 转基因小鼠内源地将 n-6 转化为 n-3 多不饱和脂肪酸 (PUFA)。本研究的目的是测试：a）鱼油喂养是否可以达到与 fat-1 小鼠相似的大脑 n-3 PUFA 水平，以及 b）fat-1 小鼠大脑二十二碳六烯酸 (22:6n-3; DHA) 水平是否可以通过鱼油喂养得到增强。Fat-1 小鼠及其野生型同窝仔鼠分别食用 10%红花油 (SO) 或 2%鱼油和 8%红花油饲料 (FO)。使用 GC-FID 分析大脑总脂质和磷脂脂肪酸。食用 FO 饲料的野生型小鼠大脑中的 DHA 水平与食用 SO 饲料的 fat-1 小鼠相似。食用 FO 饲料的 fat-1 小鼠大脑中的 n-3 PUFA 水平与食用 SO 饲料的 fat-1 小鼠相似。总之，fat-1 小鼠的大脑 DHA 水平可以通过并不能通过鱼油喂养进一步提高。
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