一种用于在不同类型基底上培养和分化胚胎干细胞的微通道-微孔系统。

A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate.

机构信息

Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, 606-8507, Japan.

出版信息

Biomed Microdevices. 2010 Jun;12(3):505-11. doi: 10.1007/s10544-010-9407-4.

PMID:20177790

Abstract

We have developed a combined micro-channel and micro-well system for easy cell loading, culture and post-culture operation on a chip. To demonstrate the reliability of the system, on chip cell culture and differentiation were performed with different types of substrates made of culture dish, glass cover slide and polydimethylsiloaxe (PDMS). As expected, mouse embryo fibroblasts (MEF) showed different adhesion and growth rate on different substrates. When embryonic stem (ES) cells were co-cultured with MEFs, the formation of ES colonies is efficient on both glass and Petri dish, although PDMS could also be used. Finally, ES cell differentiation with neuron growth factors was performed on different substrates, showing clear advantages of using culture Petri dish over both glass and PDMS.

摘要

我们开发了一种组合式微通道和微井系统，可方便地在芯片上进行细胞加载、培养和培养后操作。为了验证该系统的可靠性，我们在芯片上使用不同类型的培养皿、玻璃盖玻片和聚二甲基硅氧烷（PDMS）进行了细胞培养和分化实验。不出所料，不同类型的基质上，原代小鼠胚胎成纤维细胞（MEF）的黏附和生长速度存在差异。当胚胎干细胞（ES）与 MEF 共培养时，ES 集落的形成在玻璃和培养皿上都很有效，尽管 PDMS 也可以使用。最后，在不同的基质上进行了 ES 细胞分化与神经元生长因子实验，结果显示使用培养皿明显优于玻璃和 PDMS。