Institute of Cancer Research of Jiangsu Province, Nanjing 210009, China.
Mol Biol Rep. 2011 Jun;38(5):3181-7. doi: 10.1007/s11033-010-9989-1. Epub 2010 Feb 23.
We designed to understand the effects of the T1151A gene polymorphism in the hMLH1 gene on the pathogenesis of familial gastric cancer. Peripheral blood DNA from 113 patients with familial gastric cancer or suspected familial gastric cancer that were newly identified in the same year, along with 180 healthy subjects, was subjected to polymerase chain reaction-denaturing high-performance liquid chromatography (PCR-DHPLC) and DNA sequencing of exon 12 in the hMLH1 gene. Our results as following, the T1151A detection rate was remarkably higher in patients with familial gastric cancer or suspected familial gastric cancer compared to normal control patients (P < 0.05). Stratified analysis showed that there was a significant difference in the detection rate between the control group and elderly patients whose age of onset was greater than 50 years old (P < 0.05). The detection rate of patients from high-risk families were relatively high (P < 0.05). An especially significant distribution was observed in patients who had suffered precancerous diseases related to gastric cancer (P < 0.01). In conclusion, familial gastric carcinoma families in China carrying the T1151A polymorphism may have a higher risk of suffering from gastric cancer. This gene polymorphism can be used as a candidate screening index for high-risk populations.
我们旨在了解 hMLH1 基因中 T1151A 基因多态性对家族性胃癌发病机制的影响。对当年新确诊的 113 例家族性胃癌或疑似家族性胃癌患者和 180 例健康对照者的外周血 DNA 进行聚合酶链反应-变性高效液相色谱（PCR-DHPLC）和 hMLH1 基因第 12 外显子的 DNA 测序。结果如下，家族性胃癌或疑似家族性胃癌患者的 T1151A 检出率明显高于正常对照组（P<0.05）。分层分析显示，在年龄>50 岁的老年患者中，对照组与对照组的检出率差异有统计学意义（P<0.05）。高危家族患者的检出率相对较高（P<0.05）。在患有与胃癌相关的癌前疾病的患者中观察到特别显著的分布（P<0.01）。总之，中国携带 T1151A 多态性的家族性胃癌家族可能患胃癌的风险更高。该基因多态性可作为高危人群的候选筛查指标。
