Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Langmuir. 2010 Jun 1;26(11):8008-14. doi: 10.1021/la904903g.
Magnetic polymer particles have been used in a wide variety of applications ranging from targeting and separation to diagnostics and imaging. Current synthesis methods have limited these particles to spherical or deformations of spherical morphologies. In this paper, we report the use of stop flow lithography to produce magnetic hydrogel microparticles with a graphical code region, a probe region, and a magnetic tail region. These anisotropic multifunctional magnetic polymer particles are an enhanced version of previously synthesized "barcoded" particles (Science, 2007, 315, 1393-1396) developed for the sensitive and rapid multiplexed sensing of nucleic acids. The newly added magnetic region has acquired dipole moments in the presence of weak homogeneous magnetic fields, allowing the particles to align along the applied field direction. The novel magnetic properties have led to practical applications in the efficient orientation and separation of the barcoded microparticles during biological assays without disrupting detection capabilities.
磁性聚合物颗粒已广泛应用于靶向和分离、诊断和成像等领域。目前的合成方法将这些颗粒限制在球形或球形变形的形态。在本文中，我们报告了使用停流光刻技术生产具有图形码区域、探针区域和磁尾区域的磁性水凝胶微球。这些各向异性多功能磁性聚合物颗粒是之前为了灵敏快速地对核酸进行多重检测而合成的“编码”颗粒（Science，2007，315，1393-1396）的增强版本。在弱均匀磁场的存在下，新添加的磁性区域获得了偶极矩，使得颗粒能够沿着施加的磁场方向排列。新的磁性特性在生物测定过程中有效地实现了编码微球的定向和分离，而不会破坏检测能力。
