Department of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel.
BMC Genomics. 2010 Feb 24;11:133. doi: 10.1186/1471-2164-11-133.
Protein phosphorylation is responsible for a large portion of the regulatory functions of eukaryotic cells. Although the list of sequenced genomes of filamentous fungi has grown rapidly, the kinomes of recently sequenced species have not yet been studied in detail. The objective of this study is to apply a comparative analysis of the kinase distribution in different fungal phyla, and to explore its relevance to understanding the evolution of fungi and their taxonomic classification. We have analyzed in detail 12 subgroups of kinases and their distribution over 30 species, as well as their potential use as a classifier for members of the fungal kingdom.
Our findings show that despite the similarity of the kinase distribution in all fungi, their domain distributions and kinome density can potentially be used to classify them and give insight into their evolutionary origin. In general, we found that the overall representation of kinase groups is similar across fungal genomes, the only exception being a large number of tyrosine kinase-like (TKL) kinases predicted in Laccaria bicolor. This unexpected finding underscores the need to continue to sequence fungal genomes, since many species or lineage-specific properties may remain to be discovered. Furthermore, we found that the domain organization significantly varies between the fungal species. Our results suggest that protein kinases and their functional domains strongly reflect fungal taxonomy.
Comparison of the predicted kinomes of sequenced fungi suggests essential signaling functions common to all species, but also specific adaptations of the signal transduction networks to particular species.
蛋白质磷酸化是真核细胞大部分调节功能的基础。尽管丝状真菌的测序基因组列表迅速增加，但最近测序的物种的激酶组尚未得到详细研究。本研究的目的是应用不同真菌门中激酶分布的比较分析，并探索其与真菌进化和分类学分类的相关性。我们详细分析了 12 个激酶亚组及其在 30 个物种中的分布，以及它们作为真菌界成员分类器的潜在用途。
我们的研究结果表明，尽管所有真菌中的激酶分布相似，但它们的结构域分布和激酶组密度有可能用于对它们进行分类，并深入了解其进化起源。总的来说，我们发现激酶组的总体代表性在真菌基因组中是相似的，唯一的例外是在 Laccaria bicolor 中预测了大量酪氨酸激酶样（TKL）激酶。这一意外发现强调了需要继续对真菌基因组进行测序，因为可能仍有许多物种或谱系特异性的特性有待发现。此外，我们发现真菌物种之间的结构域组织有显著差异。我们的研究结果表明，蛋白激酶及其功能结构域强烈反映了真菌的分类学。
对已测序真菌的预测激酶组的比较表明，所有物种都具有基本的信号转导功能，但信号转导网络也针对特定物种进行了特定的适应。
