Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme-XM, Beliaghata, Kolkata 700 010, West Bengal, India.
Infect Genet Evol. 2010 May;10(4):511-6. doi: 10.1016/j.meegid.2010.02.008. Epub 2010 Feb 21.
The genus, Picobirnavirus (PBV), Spanish 'pico'='small', birna for 'bipartite RNA' genome, belongs to the family Picobirnaviridae under the proposed order Diplornavirales. PBV infections have been reported from diarrhoeic animal species and humans as well as from asymptomatic cases. The detection of Picobirnaviruses (PBVs) in diarrhoeic faecal specimens from children aged <5 years, suggestive of zoonotic transmission is being reported. 23 Picobirnavirus positive faecal specimens were detected by polyacrylamide gel electrophoresis (PAGE) and silver staining from a set of 1112 faecal specimens collected from an urban slum community in Kolkata between July and October 2007. The Picobirnaviruses showed either large profile (n=22) or small profile (n=1) for their bisegmented genomic double-stranded RNA (dsRNA). 13/23 positives were amplified by reverse transcription polymerase chain reaction (RT-PCR) as 201bp amplicon with genogroup I primers [PicoB25(+) and PicoB43(-) specific for RNA dependent RNA polymerase (RdRp) gene fragment encoded by genomic segment 2] and seven amplicons were sequenced [GPBV1-5, 7 and 8]. Sequence analyses showed that four PBV strains [GPBV1-3 and 8] resembled different clones of porcine PBV strains (D4, D6 and C10) reported in 2008 from Hungary and two PBV strains [GPBV4 and 7] resembled human PBV strains (P597, Kolkata and 2-GA-91, USA) with the maximum nucleotide (nt) identity ranging from 78% to 92%. One strain GPBV5 clustered with human PBVs and porcine PBVs that were reported from Hungary, Venezuela and Argentina showing close homology to human-like PBVs. Therefore, the close monitoring of their global spread as well as in-depth molecular characterization is essential for better understanding of emerging PBV strains.
小双 RNA 病毒（PBV）属，西班牙语“pico”意为“小”，birna 意为“双份 RNA”基因组，属于提出的双 RNA 病毒目 Diplornavirales 下的小双 RNA 病毒科。PBV 感染已在腹泻动物物种和人类以及无症状病例中报告。从 2007 年 7 月至 10 月在加尔各答一个城市贫民窟社区收集的 1112 份粪便标本中，通过聚丙烯酰胺凝胶电泳（PAGE）和银染检测到小双 RNA 病毒（PBV）呈阳性，提示存在人畜共患传播。从小双 RNA 病毒呈阳性的 23 份粪便标本中检测到 22 份大轮廓（n=22）或小轮廓（n=1）的双节段基因组双链 RNA（dsRNA）。用基因 I 组引物 [基因组 2 编码 RNA 依赖性 RNA 聚合酶（RdRp）基因片段的 PicoB25(+)和 PicoB43(-)] 通过反转录聚合酶链反应（RT-PCR）对 13/23 个阳性标本进行扩增，得到 201bp 扩增子，对 7 个扩增子进行测序[GPBV1-5、7 和 8]。序列分析表明，4 株 PBV 株[GPBV1-3 和 8]与 2008 年匈牙利报道的不同克隆猪 PBV 株（D4、D6 和 C10）相似，2 株 PBV 株[GPBV4 和 7]与人类 PBV 株（P597、加尔各答和 2-GA-91、美国）相似，最大核苷酸（nt）同一性范围为 78%至 92%。一株 GPBV5 与来自匈牙利、委内瑞拉和阿根廷的人类 PBV 和猪 PBV 聚类，与类似人类的 PBV 具有密切同源性。因此，必须密切监测其在全球的传播，并进行深入的分子特征分析，以更好地了解新兴的 PBV 株。
