• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

巴西微小双股RNA病毒（PBV）毒株的电泳RNA基因组图谱及从腹泻犊牛分离出的一株PBV的分子特征

Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf.

作者信息

Takiuchi Elisabete, Macedo Rubia, Kunz Andressa Fernanda, Gallego Jessica Cristhine, de Mello Janaina Lustosa, Otonel Rodrigo Alejandro Arellano, Alfieri Amauri Alcindo

机构信息

Department of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil.

Department of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil.

出版信息

Virus Res. 2016 Jan 4;211:58-63. doi: 10.1016/j.virusres.2015.09.022. Epub 2015 Oct 3.

DOI:10.1016/j.virusres.2015.09.022
PMID:26435337
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7127629/
Abstract

Picobirnavirus (PBV) belongs to the family Picobirnaviridae. PBV are a group of emerging non-enveloped viruses, with a bisegmented double-stranded RNA genome that can infect a wide range of hosts. This study reports the occurrence of PBV in fecal samples from five Brazilian dairy cattle herds. From the 289 stool samples of individual calves analyzed by silver-stained polyacrylamide gel electrophoresis (ss-PAGE) the PBV was detected in 8.3 % (24/289), of which 10.2% (18/176) had diarrheic consistency. Of the 24 positive samples in ss-PAGE, 5 (20.8%) of them showed a small electrophoretic profile and 19 (79.2%) samples had large profile. From the 24 positives samples by ss-PAGE, 15 (62.5%) were successfully amplified (201 bp) using GI specific primers targeting the RdRp gene of PBV. The analysis of nucleotide identity matrix revealed that the bovine PBV strain identified in this study, showed the highest nucleotide identity (81%) with PBV strain detected in turkey (MD-2010/HM803965). This is the first nucleotide sequence of a bovine PBV strain in the American continent and the first detection of small genome profile of PBV-like strains in bovine hosts.

摘要

微小双节段RNA病毒（PBV）属于微小双节段RNA病毒科。PBV是一类新出现的无包膜病毒，其基因组为双节段双链RNA，可感染多种宿主。本研究报告了巴西五个奶牛群粪便样本中PBV的存在情况。通过银染聚丙烯酰胺凝胶电泳（ss-PAGE）分析289份个体犊牛粪便样本，检测到8.3%（24/289）的样本中存在PBV，其中10.2%（18/176）的样本具有腹泻样性状。在ss-PAGE检测的24份阳性样本中，5份（20.8%）显示出小电泳图谱，19份（79.2%）样本具有大电泳图谱。在ss-PAGE检测的24份阳性样本中，15份（62.5%）使用靶向PBV RdRp基因的GI特异性引物成功扩增（201 bp）。核苷酸同一性矩阵分析显示，本研究中鉴定的牛PBV毒株与在火鸡中检测到的PBV毒株（MD-2010/HM803965）具有最高的核苷酸同一性（81%）。这是美洲大陆首次报道牛PBV毒株的核苷酸序列，也是首次在牛宿主中检测到类似PBV毒株的小基因组图谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e1c/7127629/e7229f39b0cc/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e1c/7127629/155d75747d33/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e1c/7127629/e7229f39b0cc/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e1c/7127629/155d75747d33/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e1c/7127629/e7229f39b0cc/gr2_lrg.jpg

相似文献

1
Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf.巴西微小双股RNA病毒（PBV）毒株的电泳RNA基因组图谱及从腹泻犊牛分离出的一株PBV的分子特征
Virus Res. 2016 Jan 4;211:58-63. doi: 10.1016/j.virusres.2015.09.022. Epub 2015 Oct 3.
2
Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand.泰国腹泻仔猪中微小双RNA病毒的分子检测与特性分析
Arch Virol. 2017 Apr;162(4):1061-1066. doi: 10.1007/s00705-016-3190-3. Epub 2016 Dec 28.
3
Detection of closely related Picobirnaviruses among diarrhoeic children in Kolkata: evidence of zoonoses?在加尔各答腹泻儿童中检测到密切相关的微小双 RNA 病毒：人畜共患病的证据？
Infect Genet Evol. 2010 May;10(4):511-6. doi: 10.1016/j.meegid.2010.02.008. Epub 2010 Feb 21.
4
Detection of picobirnavirus in HIV-infected patients with diarrhea in Argentina.阿根廷腹泻的HIV感染患者中微小双RNA病毒的检测
J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Aug 1;18(4):380-3. doi: 10.1097/00042560-199808010-00010.
5
Picobirnavirus detection in bovine and buffalo calves from foothills of Himalaya and Central India.喜马拉雅山山麓和印度中部地区牛犊和水牛犊中微小双RNA病毒的检测
Trop Anim Health Prod. 2011 Dec;43(8):1475-8. doi: 10.1007/s11250-011-9834-0. Epub 2011 Apr 10.
6
Genogroup I avian picobirnavirus detected in Brazilian broiler chickens: a molecular epidemiology study.巴西肉鸡中检测到的 I 型禽微小 RNA 病毒：分子流行病学研究。
J Gen Virol. 2014 Jan;95(Pt 1):117-122. doi: 10.1099/vir.0.054783-0. Epub 2013 Oct 9.
7
High detection rate and genetic diversity of picobirnavirus in a sheep flock in Brazil.巴西羊群中小肠结肠炎耶尔森菌的高检出率和遗传多样性。
Virus Res. 2018 Aug 15;255:10-13. doi: 10.1016/j.virusres.2018.06.016. Epub 2018 Jul 3.
8
Two instances of large genome profile picobirnavirus occurrence in Argentinian infants with diarrhea over a 26-year period (1977-2002).在26年期间（1977年至2002年），阿根廷腹泻婴儿中出现两例大基因组图谱微小双股RNA病毒。
J Infect. 2008 May;56(5):371-5. doi: 10.1016/j.jinf.2008.02.017. Epub 2008 Apr 9.
9
Detection and Molecular Characterization of Picobirnaviruses (PBVs) in the Mongoose: Identification of a Novel PBV Using an Alternative Genetic Code.检测貂体内的微小双核糖核酸病毒（PBVs）并对其进行分子特征分析：使用替代遗传密码鉴定新型 PBV。
Viruses. 2020 Jan 15;12(1):99. doi: 10.3390/v12010099.
10
Genogroup I picobirnavirus in diarrhoeic foals: can the horse serve as a natural reservoir for human infection?Ⅰ型微小 RNA 病毒属在腹泻驹中的流行情况：马是否能成为人类感染的天然储主？
Vet Res. 2011 Mar 17;42(1):52. doi: 10.1186/1297-9716-42-52.

引用本文的文献

1
Genetic diversity, frequency and concurrent infections of picobirnaviruses in diarrhoeic calves in Turkey.土耳其腹泻犊牛中微小 RNA 病毒的遗传多样性、频率和并发感染。
Trop Anim Health Prod. 2022 Mar 5;54(2):127. doi: 10.1007/s11250-022-03128-4.
2
Molecular epidemiology and phylogenetic analysis of bovine picobirnaviruses causing calf diarrhea, in Iran.伊朗引起犊牛腹泻的牛细小双股RNA病毒的分子流行病学和系统发育分析
Vet Res Forum. 2021;12(3):319-324. doi: 10.30466/vrf.2020.110016.2620. Epub 2021 Sep 15.
3
Epidemiologic Status of Picobirnavirus in India, A Less Explored Viral Disease.

本文引用的文献

1
Epidemiology, phylogeny, and evolution of emerging enteric Picobirnaviruses of animal origin and their relationship to human strains.动物源新兴肠道微小双股RNA病毒的流行病学、系统发育和进化及其与人类毒株的关系。
Biomed Res Int. 2014;2014:780752. doi: 10.1155/2014/780752. Epub 2014 Jul 17.
2
Genogroup I avian picobirnavirus detected in Brazilian broiler chickens: a molecular epidemiology study.巴西肉鸡中检测到的 I 型禽微小 RNA 病毒：分子流行病学研究。
J Gen Virol. 2014 Jan;95(Pt 1):117-122. doi: 10.1099/vir.0.054783-0. Epub 2013 Oct 9.
3
Simian genogroup I picobirnaviruses: prevalence, genetic diversity, and zoonotic potential.
印度微小双股RNA病毒的流行病学状况，一种较少被研究的病毒性疾病。
Open Virol J. 2018 Aug 31;12:99-109. doi: 10.2174/1874357901812010099. eCollection 2018.
4
Genetic diversity of bovine Picobirnavirus, Brazil.巴西牛微小双股RNA病毒的遗传多样性
Virus Genes. 2018 Oct;54(5):724-728. doi: 10.1007/s11262-018-1586-8. Epub 2018 Jul 9.
5
Detection of novel RNA viruses from free-living gorillas, Republic of the Congo: genetic diversity of picobirnaviruses.从刚果共和国野生大猩猩中检测新型RNA病毒：微小双RNA病毒的遗传多样性
Virus Genes. 2018 Apr;54(2):256-271. doi: 10.1007/s11262-018-1543-6. Epub 2018 Feb 23.
灵长类动物 I 型细小双病毒：流行情况、遗传多样性和人畜共患潜力。
J Clin Microbiol. 2012 Aug;50(8):2779-82. doi: 10.1128/JCM.00634-12. Epub 2012 May 23.
4
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.MEGA5：用于最大似然法、进化距离法和最大简约法的分子进化遗传学分析。
Mol Biol Evol. 2011 Oct;28(10):2731-9. doi: 10.1093/molbev/msr121. Epub 2011 May 4.
5
Picobirnavirus detection in bovine and buffalo calves from foothills of Himalaya and Central India.喜马拉雅山山麓和印度中部地区牛犊和水牛犊中微小双RNA病毒的检测
Trop Anim Health Prod. 2011 Dec;43(8):1475-8. doi: 10.1007/s11250-011-9834-0. Epub 2011 Apr 10.
6
Genogroup I picobirnavirus in diarrhoeic foals: can the horse serve as a natural reservoir for human infection?Ⅰ型微小 RNA 病毒属在腹泻驹中的流行情况：马是否能成为人类感染的天然储主？
Vet Res. 2011 Mar 17;42(1):52. doi: 10.1186/1297-9716-42-52.
7
Metagenomic analysis of the turkey gut RNA virus community.对火鸡肠道 RNA 病毒群落的宏基因组分析。
Virol J. 2010 Nov 12;7:313. doi: 10.1186/1743-422X-7-313.
8
Human picobirnaviruses identified by molecular screening of diarrhea samples.通过对腹泻样本的分子筛查鉴定出人类细小病毒。
J Clin Microbiol. 2010 May;48(5):1787-94. doi: 10.1128/JCM.02452-09. Epub 2010 Mar 24.
9
Detection of closely related Picobirnaviruses among diarrhoeic children in Kolkata: evidence of zoonoses?在加尔各答腹泻儿童中检测到密切相关的微小双 RNA 病毒：人畜共患病的证据？
Infect Genet Evol. 2010 May;10(4):511-6. doi: 10.1016/j.meegid.2010.02.008. Epub 2010 Feb 21.
10
Nomenclature proposal for picobirnavirus.微小双核糖核酸病毒命名建议。
Arch Virol. 2009;154(12):1953-4. doi: 10.1007/s00705-009-0537-z. Epub 2009 Oct 31.