Center of Excellence in Ruminant Abortion and Neonatal Mortality, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Biol Trace Elem Res. 2011 Jan;139(1):24-31. doi: 10.1007/s12011-010-8640-2. Epub 2010 Feb 24.
Thirty-six calves were used in the present study. The animals were divided equally into three groups (control, test 1, and test 2). The three groups of calves were homogeneous for parity of dams, sex, and month of birth. From 14 days of age, in the test 1 group copper as copper sulfate (Merck Co, Germany) was added to each meal of milk at a rate of 10 mg/kg of milk for 14 days and in test 2 group copper as copper sulfate was added to each meal of milk at a rate of 20 mg/kg of milk for 14 days. Blood samples were taken by jugular venipuncture using disposable syringes at 14 (before Cu supplementation), 30, 60, and 80 days of age. Anticoagulated blood was used for CBC determination. Plane tubes were used for harvesting of serum and the amounts of total serum protein, albumin, iron, and copper were measured. Calves were weighted at birth and at the end of trial (day 80) and total gain and mean daily gain were calculated. Days of treatment for ill calves were also recorded during experiment. Group (treatment) had no significant effect on the amounts of measured parameters except MCH values (p < 0.05) which were significantly lower in test 1 group than other trial groups. Age (sampling time) had significant effects on the values of most measured parameters (p < 0.05) except WBC, lymphocyte, total protein, and fibrinogen. Significant interactions between sampling time and group were not seen for any of measured parameters. No significant differences were seen for total weight gain and mean daily gain between trial groups. Chi-square test revealed no significant difference for the days of treatment between trials groups.
本研究使用了 36 头小牛。这些动物被平均分为三组（对照组、试验 1 组和试验 2 组）。这三组小牛在胎次、性别和出生月份上具有同质性。从 14 日龄开始，在试验 1 组中，每顿奶中添加硫酸铜（Merck Co，德国），剂量为 10mg/kg 奶，持续 14 天；在试验 2 组中，每顿奶中添加硫酸铜，剂量为 20mg/kg 奶，持续 14 天。在 14 天（补充 Cu 之前）、30 天、60 天和 80 天龄时，通过颈静脉穿刺使用一次性注射器采集血样。抗凝血液用于 CBC 测定。使用平面管采集血清，并测量总血清蛋白、白蛋白、铁和铜的含量。小牛在出生时和试验结束时（第 80 天）称重，并计算总增重和平均日增重。在实验过程中还记录了患病小牛的治疗天数。组（处理）对测量参数的数量没有显著影响，除了 MCH 值（p<0.05），试验 1 组明显低于其他试验组。年龄（采样时间）对大多数测量参数的值有显著影响（p<0.05），除了 WBC、淋巴细胞、总蛋白和纤维蛋白原。在任何测量参数中，均未观察到采样时间和组之间的显著相互作用。试验组之间的总增重和平均日增重没有显著差异。卡方检验显示试验组之间的治疗天数没有显著差异。
