Department of Clinical Sciences and Center of Excellence in Ruminant Abortion and Neonatal Mortality, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Food Chem Toxicol. 2010 May;48(5):1316-20. doi: 10.1016/j.fct.2010.02.030. Epub 2010 Feb 25.
Forty Holstein calves were used for the experiment and randomly assigned to four different treatments. Treatments consisted of control (injections of 5 ml normal saline), test 1 (500 mg Fe injected to each calf at 24-48 h of age), test 2 (200,000 IU vitamin A was injected to each calf at 24-48 h of age), and test 3 (Fe and vitamin A were injected to each calf as mentioned previously. Group had significant effect on the amounts of HCT, RBC, hemoglobin, MCH, platelets, iron concentration and total iron binding capacity (TIBC) saturation (p<0.05). These parameters were significantly higher in test 1 for RBC parameters, test 1 and test 3 for iron concentration and test 1 for TIBC saturation in comparing to control group. Age (sampling time) had significant effects on the values of most measured parameters (p<0.05) except MCHC, WBC, TIBC, and TIBC saturation. Significant interactions between sampling time and group were seen for HCT, RBC number, hemoglobin concentration, and MCV. No significant differences were seen for total weight gain, and mean daily weight gain between groups. Chi-square test revealed no significant difference for the percent of days of treatment between trial groups.
四十头荷斯坦犊牛用于该实验，并被随机分为四个不同的处理组。处理组包括对照组（在出生后 24-48 小时内给每头犊牛注射 5 毫升生理盐水）、试验 1 组（在出生后 24-48 小时内给每头犊牛注射 500 毫克铁）、试验 2 组（在出生后 24-48 小时内给每头犊牛注射 200000IU 维生素 A）和试验 3 组（如前所述，给每头犊牛注射铁和维生素 A）。组间差异对 HCT、RBC、血红蛋白、MCH、血小板、铁浓度和总铁结合能力（TIBC）饱和度有显著影响（p<0.05）。与对照组相比，RBC 参数中试验 1 组、铁浓度中试验 1 和试验 3 组、TIBC 饱和度中试验 1 组的这些参数显著升高。年龄（采样时间）对大多数测量参数的数值有显著影响（p<0.05），除了 MCHC、WBC、TIBC 和 TIBC 饱和度。HCT、RBC 数、血红蛋白浓度和 MCV 之间存在采样时间与组之间的显著相互作用。各组之间的总增重和平均日增重没有显著差异。卡方检验显示试验组之间的治疗天数百分比没有显著差异。
