一种针对胃肠道内镜的细菌生物膜清除效果的研究。
A study of the efficacy of bacterial biofilm cleanout for gastrointestinal endoscopes.
机构信息
Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2010 Feb 28;16(8):1019-24. doi: 10.3748/wjg.v16.i8.1019.
AIM
To compare the influence and clearance effect of enzymatic and non-enzymatic detergents against Escherichia coli (E. coli) biofilm on the inner surface of gastroscopes.
METHODS
Teflon tubes were incubated in a mixture of different detergents and E. coli culture (10(6) CFU/mL) for 72 h at 15 degrees C, and biofilms on the inner surface of the teflon tubes were analyzed by bacterial count and scanning electron microscopy. To evaluate the clearance effect of detergents, after biofilms were formed on the inner surface of Teflon tubes by 72 h lavage with E. coli culture, tubes were lavaged by enzymatic and non-enzymatic detergents at a speed of 250 mL/min, then biofilms on the inner surface were analyzed by bacterial count and scanning electron microscopy.
RESULTS
Non-enzymatic detergent had a better inhibition function on biofilm formation than enzymatic detergent as it reduced bacterial burden by 2.4 log compared with the control samples (P = 0.00). Inhibition function of enzymatic detergent was not significantly different to that of control samples and reduced bacterial burden by 0.2 log on average (P > 0.05). After lavaging at 250 mL/min for 3 min, no living bacteria were left in the tubes. Scanning electron microscopy observation showed biofilms became very loose by the high shear force effect.
CONCLUSION
Non-enzymatic detergent has a better inhibition effect on biofilm formation at room temperature. High speed pre-lavage and detergents are very important in temporal formed biofilm elimination.
目的
比较酶清洁剂和非酶清洁剂对胃内表面大肠杆菌（E. coli）生物膜的影响和清除效果。
方法
将特氟龙管置于不同清洁剂和 E. coli 培养物（10(6) CFU/mL）的混合物中，在 15°C 下孵育 72 小时，通过细菌计数和扫描电子显微镜分析特氟龙管内表面上的生物膜。为了评估清洁剂的清除效果，在特氟龙管内表面用 E. coli 培养物冲洗 72 小时形成生物膜后，用酶清洁剂和非酶清洁剂以 250 mL/min 的速度冲洗管，然后通过细菌计数和扫描电子显微镜分析管内表面的生物膜。
结果
非酶清洁剂对生物膜形成的抑制作用优于酶清洁剂，因为它使细菌负荷减少了 2.4 对数级，与对照样品相比（P = 0.00）。酶清洁剂的抑制作用与对照样品无显著差异，平均减少细菌负荷 0.2 对数级（P > 0.05）。以 250 mL/min 的速度冲洗 3 分钟后，管内没有活细菌。扫描电子显微镜观察显示，生物膜由于高剪切力的作用变得非常疏松。
结论
非酶清洁剂在室温下对生物膜形成有更好的抑制作用。高速预冲洗和清洁剂在临时形成的生物膜清除中非常重要。
Zotero 插件