Srihanam P, Simcheur W, Srisuwan Y
Department of Chemistry, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand.
Pak J Biol Sci. 2009 Nov 15;12(22):1487-90. doi: 10.3923/pjbs.2009.1487.1490.
This study aimed to prepare and characterize silk sericin and chitosan blend film as well as the native silk sericin and chitosan films. The films were observed their morphology using Scanning Electron Microscope (SEM). The secondary structures of the films were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. Transparency of the films was investigated with UV-visible spectroscopy. The results found that all of silk films were smooth throughout the film surfaces, including blend film. This showed that silk sericin and chitosan very well compatible. However, phase separation is also being observed. It is show that the interaction between two materials might be miscible together. The FTIR results indicated that the most of films were composed both in random coil and beta-sheet forms which predominantly of the random coil structures. The results suggesting the blend film between sericin and chitosan did not change the intramolecular structure when compared to the native films. The silk sericin and blend films were slightly yellowish color and were higher transparent than chitosan film. However, % transmittance at lamda max of 660 nm showed that all of films have similar values. The result suggested that the transparency of the film did not change even blend together. It is a promising that both silk sericin and chitosan would be blended into many forms for applications in specifically fields.
本研究旨在制备并表征丝胶蛋白与壳聚糖共混膜以及天然丝胶蛋白膜和壳聚糖膜。使用扫描电子显微镜（SEM）观察这些膜的形态。利用傅里叶变换红外（FTIR）光谱分析膜的二级结构。用紫外可见光谱研究膜的透明度。结果发现，所有丝膜在整个膜表面都是光滑的，包括共混膜。这表明丝胶蛋白和壳聚糖具有很好的相容性。然而，也观察到了相分离。这表明两种材料之间的相互作用可能是可混溶的。FTIR结果表明，大多数膜由无规卷曲和β-折叠形式组成，其中主要是无规卷曲结构。结果表明，与天然膜相比，丝胶蛋白与壳聚糖的共混膜并未改变分子内结构。丝胶蛋白膜和共混膜呈微黄色，比壳聚糖膜具有更高的透明度。然而，在660nm最大波长处的透光率百分比表明，所有膜的值相似。结果表明，即使混合在一起，膜的透明度也不会改变。丝胶蛋白和壳聚糖有望以多种形式混合，用于特定领域的应用。
