Watcharin Ong-chiari, Yaowalak Srisuwan, Wilaiwan Simcheur, Prasong Srihanam
Division of Secretary, Department of Chemistry, Faculty of Science, Mahasarakham University, 44150, Thailand.
Pak J Biol Sci. 2009 Dec 1;12(23):1526-30. doi: 10.3923/pjbs.2009.1526.1530.
This study aimed to prepare Silk Fibroin (SF) and Gelatin (G) blend film and study its morphology, secondary structure and thermal properties compared to native SF and G films. The films were prepared from the SF solution by casting on the polystyrene plates. They were investigated their secondary structure by fourier transform-infrared (FTIR) spectroscopy, morphology using Scanning Electron Microscope (SEM). In addition, Thermogravimetric Analysis (TG) and Differential TG (DTG) were used for thermal properties investigation. The results found that the SF/G blend film composed of both alpha-helix and beta-sheet structures which were similar characteristics of the native SF and G. This result was similar to the TG and DTG analysis according to blending between SF and G is not enhancing thermal stability of the film. However, changes in some absorption bands and temperatures were also observed from the blend film. The result suggested that chemical interaction and hydrogen bonding between SF and G could be formed. The formation could be affected to the uniform of the surface throughout the film under SEM.
本研究旨在制备丝素蛋白（SF）与明胶（G）的共混膜，并与天然SF膜和G膜相比，研究其形态、二级结构和热性能。通过在聚苯乙烯板上浇铸由SF溶液制备这些膜。利用傅里叶变换红外（FTIR）光谱研究它们的二级结构，使用扫描电子显微镜（SEM）观察形态。此外，采用热重分析（TG）和微商热重分析（DTG）研究热性能。结果发现，SF/G共混膜由α-螺旋和β-折叠结构组成，这与天然SF和G的特征相似。该结果与TG和DTG分析结果相似，即SF和G之间的共混并未提高膜的热稳定性。然而，在共混膜中也观察到一些吸收带和温度的变化。结果表明，SF和G之间可能形成了化学相互作用和氢键。这种形成可能会影响SEM下整个膜表面的均匀性。
