Prasong Srihanam, Wasan Tessanan
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Sciences, Mahasarakham University, Maha Sarakham 44150, Thailand.
Pak J Biol Sci. 2011 Mar 1;14(5):351-6. doi: 10.3923/pjbs.2011.351.356.
Abstract: Keratin solution was extracted from human hairs and used as subject for preparation of keratin/gelatin blend films. This study was aimed to explore the suitable method using for keratin extraction and extend to study the blend film properties. The blend films were prepared by simple evaporation method. After homogeneously mixed between keratin and gelatin solution at different ratios, the solution were placed on the plates and left in an oven at 40 degrees C for 3 days. All of the films were then analyzed for their morphology, secondary structures and thermal properties by using SEM, FTIR and TGA, respectively. The result from SEM images showed that native keratin films have the highest rough surface compared to other films. In addition, the smooth surface of films gradually increased when the gelatin content increased. Keratin blending with gelatin showed structural changes, especially at the absorption bands of 3300-2900 cm(-1) as well as the amide I, II and III regions. Moreover, thermal properties of the keratin films were enhanced by blending with gelatin. This study suggested that gelatin help to improve some properties of keratin while still remain its strength.
从人发中提取角蛋白溶液，并将其用作制备角蛋白/明胶共混膜的原料。本研究旨在探索合适的角蛋白提取方法，并进一步研究共混膜的性能。共混膜采用简单蒸发法制备。将角蛋白和明胶溶液按不同比例均匀混合后，将溶液置于平板上，在40℃烘箱中放置3天。然后分别使用扫描电子显微镜（SEM）、傅里叶变换红外光谱仪（FTIR）和热重分析仪（TGA）对所有薄膜的形态、二级结构和热性能进行分析。扫描电子显微镜图像结果表明，与其他薄膜相比，天然角蛋白薄膜的表面粗糙度最高。此外，随着明胶含量的增加，薄膜的光滑表面逐渐增加。角蛋白与明胶共混显示出结构变化，特别是在3300 - 2900 cm(-1)以及酰胺I、II和III区域的吸收带处。此外，通过与明胶共混，角蛋白薄膜的热性能得到了增强。本研究表明，明胶有助于改善角蛋白的一些性能，同时仍保留其强度。
