Teng Yen-Ni, Liao Ming-Hui, Lin Yang-Ben, Kuo Pao-Lin, Kuo Ting-Yi
Department of Biological Sciences and Technology, National University of Tainan, Taiwan.
Int J Androl. 2010 Dec;33(6):832-40. doi: 10.1111/j.1365-2605.2009.01038.x.
The mouse leucine-rich repeats and WD repeat domain containing 1 (lrwd1) gene is located on chromosome 5qG2 and spans over 13 kilobases. It encodes a novel protein of 648-amino acid protein that shares 78.3% amino acid sequence identity with the human LRWD1 protein. We used an oligopeptide as immunogen to generate an anti-lrwd1 antibody in rabbits. Both Northern and Western blot results indicated that the expression of lrwd1 is testis specific. Immunostaining of mouse testis sections detected high levels of lrwd1 signals in the cytoplasm of primary spermatocytes to mature spermatozoa and much weaker signals in spermatogonia. On mature spermatozoa, the anti-lrwd1 antibody stained strongly the connection region between the head and the neck where the centrosome is located. Additional immunostaining and immunoprecipitation showed colocalization and interaction between lrwd1 and γ-tubulin respectively, implicating lrwd1 as a candidate centrosomal protein. These results suggest that lrwd1 may play an important role in spermatogenesis.
小鼠富含亮氨酸重复序列和WD重复结构域1（lrwd1）基因位于5号染色体qG2上，跨越超过13千碱基对。它编码一种由648个氨基酸组成的新型蛋白质，与人类LRWD1蛋白质的氨基酸序列同一性为78.3%。我们使用一种寡肽作为免疫原在兔体内产生抗lrwd1抗体。Northern印迹和Western印迹结果均表明lrwd1的表达具有睾丸特异性。对小鼠睾丸切片进行免疫染色，在初级精母细胞至成熟精子的细胞质中检测到高水平的lrwd1信号，而在精原细胞中的信号则弱得多。在成熟精子上，抗lrwd1抗体强烈染色头部与颈部之间的连接区域，即中心体所在位置。额外的免疫染色和免疫沉淀分别显示lrwd1与γ-微管蛋白共定位和相互作用，提示lrwd1是一种候选中心体蛋白。这些结果表明lrwd1可能在精子发生中起重要作用。
