Hart P E, Glantz J N, Orth J D, Poynter G M, Salisbury J L
Tumor Biology Program, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
Genomics. 1999 Sep 1;60(2):111-20. doi: 10.1006/geno.1999.5880.
Centrin is a centrosome component in species from yeast to humans. Here, the mouse centrin 1 gene (Cetn1) is analyzed with respect to its genomic structure, chromosome localization, tissue-specific expression, and phylogenetic relationship to the other mouse centrin genes and their human orthologs. Cetn1 is an intronless gene located on chromosome 18A2 that encodes a 172-amino-acid protein with a predicted molecular mass of 19,696 Da (pI 4.61) and all of the structural features common to centrin. Cetn1 possesses the sequence features of an expressed retroposon: the gene lacks introns, the open reading frame is not interrupted by stop codons, and the coding region is flanked by a pair of direct repeats. Reverse transcriptase-polymerase chain reaction and Northern blot analysis demonstrate that Cetn1 expression is limited exclusively to the testis in adult male mice. Cetn1 expression is first seen in the neonatal testis at 14 days postpartum, reaching adult levels by day 17. These observations provide new insight into the regulation, function, and evolutionary history of centrin in higher eukaryotes.
中心蛋白是从酵母到人类等物种中的一种中心体成分。在此，对小鼠中心蛋白1基因（Cetn1）进行了分析，涉及其基因组结构、染色体定位、组织特异性表达，以及与其他小鼠中心蛋白基因及其人类直系同源基因的系统发育关系。Cetn1是一个无内含子基因，位于18A2染色体上，编码一种172个氨基酸的蛋白质，预测分子量为19,696 Da（pI 4.61），具有中心蛋白共有的所有结构特征。Cetn1具有一个已表达反转录转座子的序列特征：该基因没有内含子，开放阅读框未被终止密码子打断，编码区两侧有一对同向重复序列。逆转录-聚合酶链反应和Northern印迹分析表明，Cetn1的表达在成年雄性小鼠中仅局限于睾丸。Cetn1的表达在产后14天的新生睾丸中首次出现，到第17天达到成年水平。这些观察结果为高等真核生物中中心蛋白的调控、功能和进化史提供了新的见解。
