Driguez Patrick, Doolan Denise L, Loukas Alex, Felgner Philip L, McManus Donald P
Molecular Parasitology Laboratory, The Queensland Institute of Medical Institute, Herston, Queensland 4006, Australia.
Parasit Vectors. 2010 Jan 28;3:4. doi: 10.1186/1756-3305-3-4.
The recent publication of the Schistosoma japonicum and S. mansoni genomes has expanded greatly the opportunities for post-genomic schistosomiasis vaccine research. Immunomics protein microarrays provide an excellent application of this new schistosome sequence information, having been utilised successfully for vaccine antigen discovery with a range of bacterial and viral pathogens, and malaria.Accordingly, we have designed and manufactured a Schistosoma immunomics protein microarray as a vaccine discovery tool. The microarray protein selection combined previously published data and in silico screening of available sequences for potential immunogens based on protein location, homology to known protective antigens, and high specificity to schistosome species. Following cloning, selected sequences were expressed cell-free and contact-printed onto nitrocellulose microarrays. The reactivity of microarray proteins with antisera from schistosomiasis-exposed/resistant animals or human patients can be measured with labelled secondary antibodies and a laser microarray scanner; highly reactive proteins can be further assessed as putative vaccines. This highly innovative technology has the potential to transform vaccine research for schistosomiasis and other parasitic diseases of humans and animals.
日本血吸虫和曼氏血吸虫基因组的近期公布极大地拓展了基因组学时代血吸虫病疫苗研究的机遇。免疫组学蛋白质微阵列很好地应用了这些新的血吸虫序列信息，已成功用于多种细菌、病毒病原体及疟疾的疫苗抗原发现。因此，我们设计并制作了一种血吸虫免疫组学蛋白质微阵列作为疫苗发现工具。微阵列蛋白质的选择结合了先前发表的数据以及对可用序列进行的基于蛋白质定位、与已知保护性抗原的同源性和对血吸虫物种的高特异性的潜在免疫原的电子筛选。克隆后，将选定的序列进行无细胞表达，并接触印刷到硝酸纤维素微阵列上。微阵列蛋白质与来自血吸虫病暴露/抗性动物或人类患者的抗血清的反应性可以用标记的二抗和激光微阵列扫描仪进行检测；高反应性蛋白质可作为推定疫苗进行进一步评估。这项极具创新性的技术有可能改变血吸虫病以及其他人类和动物寄生虫病的疫苗研究。
