Rozak David A, Gelhaus Herbert C, Smith Mark, Zadeh Mojgan, Huzella Louis, Waag David, Adamovicz Jeffrey J
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
J Immune Based Ther Vaccines. 2010 Feb 5;8(1):2. doi: 10.1186/1476-8518-8-2.
Studies have shown that CpG oligodeoxyribonucleotides (ODN) protect mice from various bacterial pathogens, including Burkholderia pseudomallei and Francisella tularensis live vaccine strain (LVS), when administered before parenteral challenge. Given the potential to develop CpG ODN as a pre-treatment for multiple bacterial biological warfare agents, we examined survival, histopathology, and cytokine data from CpG ODN-treated C57BL/6 mice to determine whether previously-reported protection extended to aerosolized B. pseudomallei 1026b and highly virulent F. tularensis Schu S4 infections. We found that, although CpG ODN protected mice from aerosolized B. pseudomallei challenges, the immunostimulant failed to benefit the animals exposed to F. tularensis Schu S4 aerosols. Our results, which contrast with earlier F. tularensis LVS studies, highlight potential differences in Francisella species pathogenesis and underscore the need to evaluate immunotherapies against human pathogenic species.
研究表明，在经肠胃外途径进行攻击之前给予小鼠CpG寡脱氧核糖核苷酸（ODN），可保护小鼠免受包括伯克霍尔德菌和土拉热弗朗西斯菌活疫苗株（LVS）在内的多种细菌病原体的侵害。鉴于有潜力将CpG ODN开发为针对多种细菌生物战剂的预处理方法，我们检查了经CpG ODN处理的C57BL/6小鼠的存活情况、组织病理学和细胞因子数据，以确定先前报道的保护作用是否也适用于雾化的伯克霍尔德菌1026b和高毒力的土拉热弗朗西斯菌Schu S4感染。我们发现，尽管CpG ODN可保护小鼠免受雾化的伯克霍尔德菌攻击，但这种免疫刺激剂未能使暴露于土拉热弗朗西斯菌Schu S4气溶胶的动物受益。我们的结果与早期关于土拉热弗朗西斯菌LVS的研究形成对比，突出了弗朗西斯菌属发病机制的潜在差异，并强调了评估针对人类致病菌种的免疫疗法的必要性。
