生成 Merkel 细胞多瘤病毒（MCV）样颗粒及其在 MCV 抗体检测中的应用。
Generation of Merkel cell polyomavirus (MCV)-like particles and their application to detection of MCV antibodies.
机构信息
INSERM U618, Faculté de Pharmacie Ph Maupas, Université François Rabelais, and Service d'Anatomie et Cytologie Pathologiques, Hôpital Trousseau, CHRU de Tours, 31 Avenue Monge, 37200 Tours, France.
出版信息
J Clin Microbiol. 2010 May;48(5):1767-70. doi: 10.1128/JCM.01691-09. Epub 2010 Feb 24.
Abstract
The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Viruslike particles (VLPs) were obtained with only one of the three VP1 genes. High-titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs, and limited cross-reactivity was observed with BK polyomavirus (BKV) and lymphotropic polyomavirus (LPV). MCV antibodies were detected in 77% of the general population, with no variations according to age.
摘要
最近有报道称，一种新的人类多瘤病毒，即 Merkel 细胞多瘤病毒（MCV），已整合到 Merkel 细胞癌（MCC）的细胞 DNA 中，MCC 是一种罕见的人类皮肤癌。为了调查普通人群中 MCV 的血清流行率，我们使用重组杆状病毒在昆虫细胞中表达了三种不同的 MCV VP1。只有三种 VP1 基因中的一种能够获得病毒样颗粒（VLPs）。用 MCV VLPs 免疫的小鼠中检测到针对 VP1 VLPs 的高滴度抗体，并且与 BK 多瘤病毒（BKV）和淋巴嗜性多瘤病毒（LPV）有有限的交叉反应性。MCV 抗体在普通人群中的检出率为 77%，且与年龄无关。
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