University of Innsbruck, Institute of Microbiology, Technikerstrasse 25d, 6020 Innsbruck, Austria.
Water Sci Technol. 2010;61(4):1003-9. doi: 10.2166/wst.2010.016.
Large waste water treatment plants (WWTP) often operate nitrification in two different process environments: the cold-dilute sewage is treated in the mainstream nitrification/denitrification system, while the high strength ammonia liquors from sludge dewatering are treated in a separate high temperature reactor (SBR). This study investigates transfer from nitrifier biomass into a two-stage WWTP, commonly referred to as bioaugmentation. Besides the quantitation of ammonia oxidising bacteria (AOB), community differences were analysed with two techniques, denaturing gradient gel electrophoresis and real-time PCR melt curve analysis. It was shown that, without bioaugmentation, two distinct AOB communities establish in the mainstream and in the SBR, respectively. A gradual shift of the two AOB communities with increasing pump rates between the systems could be demonstrated. These molecular findings support process engineering experience, that cycling of waste activated sludge improves the ability of AOB to adapt to different process environments.
大型废水处理厂（WWTP）通常在两种不同的工艺环境中进行硝化作用：冷稀污水在主流硝化/反硝化系统中处理，而来自污泥脱水的高浓度氨液则在单独的高温反应器（SBR）中处理。本研究调查了从硝化生物量转移到两级 WWTP 的情况，通常称为生物增强。除了定量氨氧化菌（AOB）外，还使用两种技术分析了群落差异，即变性梯度凝胶电泳和实时 PCR 熔解曲线分析。结果表明，在没有生物增强的情况下，AOB 分别在主流和 SBR 中建立了两个不同的群落。可以证明，随着系统之间的泵速增加，两个 AOB 群落逐渐转移。这些分子发现支持了工艺工程经验，即循环使用废活性污泥可提高 AOB 适应不同工艺环境的能力。
