Animal Genetic Improvement Division, National Institute of Animal Science, Cheonan, 330-801, Korea.
Mol Biol Rep. 2010 Oct;37(7):3621-4. doi: 10.1007/s11033-010-0012-7. Epub 2010 Feb 25.
This study was aimed to search genetic variants for the swine nerve growth factor gene that associated with regulation of proliferation and differentiation of nervous systems. The swine nerve growth factor gene was screened with 5 primer sets for random populations of crossbred pigs born 2005-2007 at National Institute of Animal Science (NIAS). To verify genetic variants of miniature pigs, a total of 288,000 BAC clones generated from NIAS in 2007 were used. The selection of primer sequences was based on sequences of the swine in GenBank (L31898), and genetic variants have been discovered in the crossbred population positioned at 381 (A/C), 412 (C/T), 422 (G/A), 468 (G/C), 496 (A/G), 538 (T/C), 540 (G/A), and 547 (A/G) showing substitutions of amino acids. The identified sequences of miniature pigs including SNPs were submitted into GenBank with an accession number (GQ423508). The sequence alignment conducted to compare genetic distances between species, revealing not many high similarities between swine and human as approximately 0.89 that was a little bit high value than expected. Consequently, we suggest that the identified SNPs of the swine NGF gene may be used in the future to identify genetic markers in coding regions, regarding explanations of phenotypic variations.
本研究旨在寻找与神经系统增殖和分化调节相关的猪神经生长因子基因的遗传变异。使用 5 个引物组对 2005-2007 年出生于国立动物科学研究所（NIAS）的杂交猪随机群体进行了猪神经生长因子基因的筛选。为了验证小型猪的遗传变异，共使用了 2007 年 NIAS 生成的 288000 个 BAC 克隆。引物序列的选择基于 GenBank 中猪的序列（L31898），在位于 381（A/C）、412（C/T）、422（G/A）、468（G/C）、496（A/G）、538（T/C）、540（G/A）和 547（A/G）的杂交群体中发现了遗传变异，这些变异导致氨基酸取代。包括 SNP 在内的小型猪鉴定序列已被提交到 GenBank，注册号为（GQ423508）。进行序列比对以比较物种之间的遗传距离，结果显示猪和人类之间的相似度不高，约为 0.89，略高于预期。因此，我们建议猪 NGF 基因的鉴定 SNP 可能用于未来识别编码区域的遗传标记，以解释表型变异。
