Department of Physics, University of Surrey, Guildford, Surrey, UK.
Biofouling. 2010 May;26(4):387-97. doi: 10.1080/08927011003646809.
The adhesion of Staphylococcus epidermidis, Escherichia coli, and Candida albicans on mucin coatings was evaluated to explore the feasibility of using the coating to increase the infection resistance of biomaterials. Coatings of bovine submaxillary mucin (BSM) were deposited on a base layer consisting of a poly(acrylic acid-b-methyl methacrylate) (PAA-b-PMMA) diblock copolymer. This bi-layer system exploits the mucoadhesive interactions of the PAA block to aid the adhesion of mucin to the substratum, whereas the PMMA block prevents dissolution of the coating in aqueous environments. The thickness of the mucin coating was adjusted by varying the pH of the solution from which it was deposited. Thin mucin coatings decreased the numbers of bacteria but increased the numbers of C. albicans adhering to the copolymer and control surfaces. Increasing the mucin film thickness resulted in a further lowering of the density of adhering S. epidermidis cells, but it did not affect the density of E. coli. In contrast, the density of C. albicans increased with an increase in mucin thickness.
评估了表皮葡萄球菌、大肠杆菌和白色念珠菌在粘蛋白涂层上的黏附情况，以探索使用涂层来提高生物材料抗感染能力的可行性。牛颌下腺粘蛋白（BSM）涂层沉积在由聚（丙烯酸-b-甲基丙烯酸甲酯）（PAA-b-PMMA）两亲嵌段共聚物组成的基层上。该双层体系利用 PAA 嵌段的粘膜黏附相互作用来帮助粘蛋白黏附到基底上，而 PMMA 嵌段则防止涂层在水介质中溶解。通过改变沉积溶液的 pH 值来调整粘蛋白涂层的厚度。薄的粘蛋白涂层减少了细菌的数量，但增加了黏附在共聚物和对照表面上的白色念珠菌的数量。增加粘蛋白膜的厚度会导致黏附表皮葡萄球菌细胞的密度进一步降低，但不会影响大肠杆菌的密度。相比之下，粘蛋白厚度的增加会导致白色念珠菌的密度增加。
