BioLocus A/S, DK 2970 Hørsholm, Denmark.
Biofouling. 2009 Oct;25(7):667-74. doi: 10.1080/08927010903096008.
The antifouling (AF) potential of the serine protease Esperase HPF (subtilisin) was evaluated for the ability to prevent the formation of a four-species bacterial biofilm. The effects of enzyme activity, time and application of the enzyme were tested on the density and the oxidative metabolism of biofilm developed in microtiter wells. Esperase HPF did not inhibit the oxidative metabolism of the bacterial biofilm or planktonic growth, but the enzyme inhibited biofilm formation by its proteolytic activity as inactivated enzyme had no effect. The effective enzyme concentration was determined over a period of 72 h, as by then all the tested concentrations inhibited biofilm formation maximally. The effective concentrations of the enzymes in solution were the same regardless of time of application (ie before or after biofilm formation), but immobilisation of the enzymes caused a lower effective concentration. Esperase HPF is an attractive alternative to the biocidal compounds used in AF coatings today.
研究了丝氨酸蛋白酶 Esperase HPF（枯草杆菌蛋白酶）的防污（AF）潜力，以评估其抑制四种细菌生物膜形成的能力。测试了酶活性、时间和酶的应用对微滴定孔中形成的生物膜密度和氧化代谢的影响。Esperase HPF 不会抑制细菌生物膜的氧化代谢或浮游生长，但酶通过其蛋白水解活性抑制生物膜形成，因为失活酶没有影响。在 72 小时的时间内确定了有效酶浓度，因为到那时，所有测试浓度都最大程度地抑制了生物膜形成。在溶液中的有效酶浓度与施加时间（即在生物膜形成之前或之后）无关，但酶的固定化会导致较低的有效浓度。Esperase HPF 是当今用于 AF 涂料的杀菌化合物的有吸引力的替代品。
