Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne-Sud, UEB, Lorient, France.
Biofouling. 2011 Sep;27(8):931-40. doi: 10.1080/08927014.2011.611878.
Bacterial biofilms occur on all submerged structures in marine environments. The authors previously reported that the marine bacterium Pseudoalteromonas sp. 3J6 secretes antibiofilm activity. Here, it was discovered that another Pseudoalteromonas sp. strain, D41, inhibited the development of strain 3J6 in mixed biofilms. Confocal laser scanning microscope observations revealed that the culture supernatant of strain D41 impaired biofilm formation of strain 3J6 and another marine bacterium. A microtiter plate assay of the antibiofilm activity was set up and validated with culture supernatants of Pseudoalteromonas sp. 3J6. This assay was used to determine the spectra of action of strains D41 and 3J6. Each culture supernatant impaired the biofilm development of 13 marine bacteria out of 18. However, differences in the spectra of action and the physical behaviours of the antibiofilm molecules suggest that the latter are not identical. They nevertheless share the originality of being devoid of antibacterial activity against planktonic bacteria.
细菌生物膜存在于海洋环境中所有的水下结构物上。作者先前报道称海洋细菌假交替单胞菌 3J6 分泌抗生物膜活性物质。在此，研究发现另一种假交替单胞菌菌株 D41 抑制了混合生物膜中 3J6 菌株的生长。激光共聚焦显微镜观察显示，D41 菌株的培养液上清液破坏了 3J6 菌株和另一种海洋细菌的生物膜形成。建立了微板检测抗生物膜活性的方法，并对假交替单胞菌 3J6 的培养液上清液进行了验证。该方法用于确定 D41 和 3J6 菌株的作用谱。每种培养液上清液都能抑制 18 株海洋细菌中的 13 株的生物膜发育。然而，作用谱和抗生物膜分子的物理行为的差异表明，它们并不相同。尽管如此，它们都具有一个共同点，即对浮游细菌没有抗菌活性。
