Li Shuai, Shi Yan, Shang Xiao-Ya, Cui Bao-Song, Yuan Yi, Chen Xiao-Guang, Yang Yong-Chun, Shi Jian-Gong
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
J Asian Nat Prod Res. 2009 Jul;11(7):652-7. doi: 10.1080/10286020902964248.
Two new triterpenoids taraxer-14-ene-1alpha,3beta-diol (1) and 3beta-hydroxytaraxer-14-ene-1-one (2), together with the known triterpenes taraxerol (3), betulin (4), betulinic acid (5), sumaresinolic acid (6), and 5-hydroxy-2-methoxy-1,4-naphthoquinone (7), 5,7-dihydroxy-6,8-dimethylchromone (8), alpha-monpalmitin (9), palmitic acid (10), 6beta-hydroxystigmast-4-en-3-one (11), beta-sitostero1 (12), have been isolated from the petroleum ether fraction of the ethanolic extract of Pterospermum heterophyllum. Their structures were established by spectroscopic methods including IR, MS, 1D, and 2D NMR experiments. Compounds 1-8 were evaluated against several human cancer cell lines. Compound 1 showed in vitro selective cytotoxicity against human lung cancer cell lines (A549) with an IC(50) value of 1.22 microM. Compound 7 showed significant cytotoxicity against the A549, HCT-8, Bel7402, BGC-823, and A2780 cancer cell lines with IC(50) values of 0.21, 0.55, 0.40, 0.59, and 0.34 microM, respectively. However, the other compounds were inactive (IC(50)>10 microM).
从异叶翅子树乙醇提取物的石油醚部分分离出两种新的三萜类化合物蒲公英赛-14-烯-1α,3β-二醇（1）和3β-羟基蒲公英赛-14-烯-1-酮（2），以及已知的三萜类化合物蒲公英赛醇（3）、桦木醇（4）、桦木酸（5）、苏门树脂酸（6）、5-羟基-2-甲氧基-1,4-萘醌（7）、5,7-二羟基-6,8-二甲基色原酮（8）、α-单棕榈酸甘油酯（9）、棕榈酸（10）、6β-羟基豆甾-4-烯-3-酮（11）、β-谷甾醇（12）。通过包括红外光谱、质谱、一维和二维核磁共振实验在内的光谱方法确定了它们的结构。对化合物1-8针对几种人类癌细胞系进行了评估。化合物1对人肺癌细胞系（A549）表现出体外选择性细胞毒性，IC(50)值为1.22微摩尔。化合物7对A549、HCT-8、Bel7402、BGC-823和A2780癌细胞系表现出显著的细胞毒性，IC(50)值分别为0.21、0.55、0.40、0.59和0.34微摩尔。然而，其他化合物无活性（IC(50)>10微摩尔）。
